欧瑞血活素注射液对小鼠免疫功能的影响

Effect of Blood Acting Polypeptide Injection on immunocompetence in mice

目的 :观察欧瑞血活素注射液对小鼠免疫功能的影响。方法 :以腹腔注射方式给予昆明小鼠不同剂量的欧瑞血活素注射液 30d ,并以生理盐水腹腔注射为阴性对照 ,干扰素腹腔注射为阳性对照。结果 :欧瑞血活素注射液小、中剂量组均具有提高中性粒细胞的杀伤功能 (P <0 .0 5 ,P <0 .0 1) ;同等剂量组还可以显著地提高小鼠溶血素水平 (P <0 .0 5 ) ;小、中、大剂量组均具有提高碳粒廓清指数和吞噬指数的作用、显著地增加脾脏及胸腺的重量 ,其中以中剂量组增强单核巨噬细胞的吞噬功能、大剂量组增加脾脏重量、中剂量组增加胸腺重量的作用最为显著。结论 :小、中剂量欧瑞血活素注射液可以提高昆明小鼠的特异性免疫、非特异性免疫以及中性粒细胞的吞噬功能。

OBJECTIVE: Investigate the effect of BAP Injection on immunocompetence in mice.METHOD:The different doses of BAP were given to Kunming mice for 30 days, ip, continuously, and the negative control group was ip NS, the positive control group was ip INF. RESULTS:(1)Among the different doses of BAP tested (low dose: 0.05ml·10g 1 ·d 1 , medium dose: 0.10 ml·10g 1 ·d 1 , high dose: 0.20 ml·10g 1 ·d 1 ), the low and medium could increase the killing function of neutrophil in mice ( P <0.05, P <0.01 vs.control). (2)The low and medium doses of HC 50 showed significant difference compared with NS control group ( P <0.05). (3)All doses could increase carbon clearance index and phagocytose index, low and medium doses showed significant difference with control group ( P <0.05, P <0.01). The high dose could profoundly raise spleen index ( P <0.01), and medium dose could largely raise thymus index ( P <0.01). CONCLUSION:BAP has somewhat adjusting effects on specific immunocompetence, nonspecific immunocompetence and killing function of neutrophil in mice, the low and medium doses have beneficial effect.