片仔癀对舌鳞状细胞癌Tca8113细胞生物学行为的抑制作用

Pien Tze Huang Inhibits the Biological Behavior of Tongue Squamous Carcinoma Tca8113 Cells

目的探讨中药片仔癀对舌鳞状细胞癌细胞生物学行为的影响及分子机制。方法将不同浓度的片仔癀作用于Tca8113细胞,应用MTT法检测Tca8113细胞的增殖情况,应用碘化丙啶(PI)染色检测细胞周期的变化,应用Western blotting检测细胞中信号转导子与转录激活子3磷酸化蛋白(p-STAT3)、Bcl-2蛋白表达的变化。结果 MTT实验结果显示,片仔癀呈剂量依赖性抑制Tca8113细胞的增殖。PI染色结果显示,片仔癀使细胞周期在G0/G1期发生阻滞。Western blotting结果提示,片仔癀抑制STAT3蛋白的磷酸化,抑制Bcl-2蛋白表达。结论片仔癀对舌鳞状细胞癌细胞的增殖具有抑制作用。

Objective To investigate the effects of Pien Tze Huang on the biological behavior of tongue squamous carcinoma Tca8113 cells and its molecular mechanism. Methods Tca8113 cells were incubated with Pien Tze Hnang at different doses. The prolifera- tion of Tca8113 cells was examined by MT]＂ assay. The distribution of the cell cycle was evaluated by PI staining. The expression of the p-STAT3 and Bcl-2 proteins was assessed by Western blotting. Results MTT assay showed that Pien Tze Huang inhibited the prolifer- ation of Tca8113 cells in a dose-dependent manner. PI staining showed that Pien Tze Huang arrested cells in the G0/G1 phase. The results of Western blotting suggested that Pien Tze Huang inhibited STAT3 protein phosphorylation and Bcl-2 protein expression. Conclu- sion Pien Tze Huang inhibits the proliferation of tongue squamous carcinoma cells.