东北地区GLIS3基因多态性与2型糖尿病合并视网膜病变的关联性研究

Polymorphisms in GLIS3 Associated with Diabetic Retinopathy in Patients with Type 2 Diabetes in Northeast China

目的探讨GLIS3基因多态性与中国东北地区2型糖尿病合并视网膜病变的相关性。方法采用病例对照研究方法,选取糖尿病合并视网膜病变患者(DR组)、糖尿病未合并视网膜病变患者(DNR组)、健康人群(NC组)各120例为研究对象。应用PCRRFLP方法测定GLIS3基因rs7041847与rs7034200多态的基因分型。用非条件logistic回归计算比值比及95%置信区间。结果 DR、DNR组分别与NC组比较,GLIS3基因rs7041847多态AG、AG/GG基因型、G等位基因患者发病风险显著增加(P<0.05),rs7034200多态CC、AC/CC基因型、C等位基因患者发病风险显著增加(P<0.05)。结论在中国东北地区,GLIS3基因rs7041847、rs7034200是2型糖尿病的危险因素,并未发现GLIS3基因与视网膜病变的易感性相关。

Objective To investigate the correlation between the polymorphisms in GLIS3 and diabetic retinopathy in patients with type 2 diabetes in Northeast China. Methods Based on the case-control study,polymorpbisms in GLISS were examined by PCR-RFLP in 120 cases of diabetic retinopathy in patients with type 2 diabetes （DR）, 120 cases of patients with type 2 diabetes without diabetic retinopa- thy （DNR）, and 120 healthy individuals （NC）. The odds ratio （OR） and 95% confidence interval （CI） were calculated using unconditional logistic analysis. Results The resuhs demonstrated that GLIS3 rs7041847 AG,AG/GG genotype,or G allele was associated with an increased risk of DR and DNR （P 〈 0.05）. Moreover, GLIS3 rs7034200 CC, AC/CC genotype, or C allele was associated with an increased risk of DR and DNR （P 〈 0.05）. Conclusion In the patients in Northeast China, the polymorphisms in GLIS3 rs7041847 and rs7034200 were correlated with type 2 diabetes mellitus, while GLIS3 may not be associated with the susceptibility to diabetic retinopathy.