药物的紫外百分吸收系数用于HPLC定量测定的方法探讨

The Determination of Drugs Contents By HPLC With E _(1cm)^(1%)

目的 :解决HPLC定量测定中需要对照品的问题。方法 :对不同的流动相及组成、比例、PH、药物性质对记录仪的响应因子进行研究。结果 :对一定的仪器、柱子和记录仪而言 ,K为一常数。结论 :以药物的紫外百分系数E1% 1cm可直接进行HPLC定量测定的方法 (C =SF/KE1%1cmV)。以本法测定复方新诺明中的SMZ、TMP含量 ,与文献方法比较 。

OBJECTIVE:To determine drug contents by HPLC with E 1% 1cm . METHOD:Study the influnce of the kinds, ratio, pH of mobile phase and properities of drugs on the respond factors of recorder. RESULTS:K is a constant for a certain instrument, column and recorder. CONCLUSION:Drugs content can be determinated by HPLC with E 1% 1cm directly.(C=SF/KE 1% 1cm V)?There is no difference when determining SMZ, TMP in SMZ, compared with reference.