高效液相色谱法同时测定复方氨酚烷胺胶囊中3种组分的含量

HPLC determination of three component in Compound Paracetamol and Amantadine Hydrochloride Capsules

目的 :采用高效液相色谱法同时测定复方氨酚烷胺胶囊中对乙酰氨基酚、咖啡因和马来酸氯苯那敏的含量。方法 :使用C18柱 (Eclipse4 .6mm× 15 0mm) ,柱温 30℃ ,流动相 :乙腈 pH3.0缓冲液 (15 :85 ) ;检测波长 2 14nm ;流速 1ml/min。 结果 :采用高效液相色谱法测定对乙酰氨基酚、咖啡因和马来酸氯苯那敏的含量 ,线性范围分别为 2 0 0～ 12 0 0 ,12 .2 8～ 73.6 8,1.0 5～ 6 .30 μg/ml,r =0 .9999;平均回收率分别为 99.8% (RSD =0 .6 % )、10 0 .0 % (RSD =0 .3% )、98.4 % (RSD =0 .9% )。结论 :本法简便快速准确 ,用高效液相色谱法同时测定复方氨酚烷胺胶囊中三个组分的含量 。

OBJECTIVE:To establish a HPLC method for the determination of paracetamol and caffeine and chlorphenamine maleate in Compound Paracetamol and Amantadine Hydrochloride Capsules. METHOD:A C 18 column (Eclipse4.6mm×150mm ), a column temperature of 30℃,a mobil phase somposed of acetonitrile and a pH3.0 buffer solution (15:85) were adopted. Wave length:214nm;flow rate1ml/min. RESULTS:Paracetamol, caffeine and chlorphenamine maleate were assayed by this method.The linear range were 200～1200,12.28～73.68 and 1.05～6.30μg/ml, respectively, r=0.9999; The recovery were 99.8%(RSD=0.6%)?100.0%(RSD=0.3%) and 98.4%(RSD=0.9%). CONCLUSION:The method is simple and rapid. HPLC determination of three component in Compound Paracetamol and Amantadine Hydrochloride Capsules can be used to control product quality preferably.