不同分子量透明质酸钠防止屈肌腱粘连作用的比较

Protecting Flexor Tendon from Adhering by Sodium Hyaluronate Products with Different Molecular Weight

目的 :以鸡爪为动物模型 ,研究不同分子量的透明质酸钠 (Sodiumhyaluronateproduct;以下简称SHP) ,对防止肌腱粘连的不同作用。方法 :72只莱亨鸡分成实验组A、B、C和对照组D ,每组又根据术后时间不同分为 3周、5周、8周三组 (n =6 ) ;实验手术斜坡形横断左足第Ⅲ趾屈趾深肌腱 ,鞘管内注射不同分子量的SHP ,A组分子量为 2 5 0万 ,B组为 10 0万 ,C组为 5 0万 ,对照组为生理盐水。结果 :经生物力学测定和粘连范围病理组织学评分 ,实验组A、B与对照组D有显著性差异 (P <0 .0 1) ,而实验组C和对照组D无显著性差异 (P≥ 0 .0 1)。结论 :不同分子量的SHP防止肌腱粘连的作用不同 ;分子量大于 10 0万的SHP具有防止粘连的作用 ;分子量为 5 0万的SHP和生理盐水作用相似 。

OBJECTIVE:To explore the effect of sodium hyaluronate products (SHPs) with different molecular weight (Mol Wt) in preventing adhesion formation in a surgically prapared chicken flexor tendon model. METHOD:72 chickens were divided into four groups: group A,B,C were study groups and group D was used as a control. Each group was further divided into three subgroups 3 week group,5 week group and 8 week group based on the postoperative time. For intrathecal injection with SHPs the deep muscle tendon in the chicken's third digit were transected. The chickens in group A, B, C were injected with SHPs with the Mol Wt of 2.5 million ,1 million and 0.5 million , respectively , and those in the control group were injected with saline.RESULTS:Adhesion scopes measured by pathologically histological scoring in group A and B were not wider than those in the control group ( P <0.01), whereas those in group C were not different to the control group( P ≥0.01). CONCLUSION:SHPs with different Mol Wt had different effects on adhesion of chicken (muscle) tendon . SHPs with Mol Wt equal to or more than 1 million protected chicken' s tendon from adhering , whereas SHP with molecular weight of 0.5 million did not , like saline.