应用齿贝骨粉及矿化胶原膜修复小型猪拔牙创骨缺损的效果观察

Repair of bone defect by using mineralized collagen materials and collagen membrane in minipig models

目的:比较Bio-Oss骨粉和齿贝骨粉修复拔牙窝骨缺损的成骨效果,评估齿贝牙科骨粉在拔牙窝骨缺损修复中的可靠性。方法:以引导骨再生术(GBR)为基本原理,在小型猪拔牙区制造骨缺损,分别植入齿贝牙科骨粉及Bio-Oss骨粉。术后12周进行肉眼观察、影像学检查及组织学染色。影像学检查测量骨缺损的成骨密度,采用SPSS 22.0软件包进行配对t检验,观察骨缺损修复情况。分析组织切片中新生骨在骨缺损区域所占面积的百分比,得出新生骨百分率,比较2种不同材料骨粉修复拔牙窝骨缺损的效果。结果:术后12周,CT显示不同组的拔牙窝骨缺损完全被新生骨填充,2种骨粉修复骨缺损效果无显著差异。组织学切片显示,与Bio-Oss骨粉相比,齿贝骨粉填充骨缺损区成骨量略有减少,但两者差异不显著。结论:齿贝牙科骨粉在拔牙窝骨缺损修复中具有可靠性,有必要做进一步临床研究。

PURPOSE： The aim of this study was to compare the clinical and histologic outcomes of bone regeneration using two different graft materials： deproteinized bovine bone mineral （Bio-Oss） and mineralized collagen bone materials （ChiBei） with absorbable collagen membrane, thus to evaluate the effect of ChiBei on bone regeneration. METHODS： The molars of the minipig were extracted, a 10mm×10mm bone defect was created. Bone defect was filled with Bio-Oss and ChiBei respectively and with absorbable collagen membrane as well. Cone-beam CT （CBCT） was taken 12 weeks after surgery and bone samples were stained with Goldner＇s Trichrome. The new regenerated area was calculated by the image analysis software Image-Pro plus 6.0 and the percentages of newly formed bone were compared between two groups using SPSS 22.0 software package. RESULTS： Bone defect was filled with new regenerated bone in each group. There was no significant difference in bone density between the ChiBei and Bio-Oss group on CBCT, so did the histologic analysis results. CONCLUSIONS： Based on the findings of the study, we speculated ChiBei materials covered with collagen membrane have positive effect on bone regeneration after tooth extraction, but more researches need to be carried out.