猪跟腱胶原蛋白酶法提取工艺的研究

Study on Preparation of Pigs' Tendon Collagen by Enzymatic Hydrolysis

为更好利用猪跟腱资源、提高跟腱的附加值,探讨猪跟腱酶法提取胶原蛋白的最佳工艺条件。采用单因素试验法考察猪跟腱脱脂时间,酶法提取工艺中胃蛋白酶用量、提取时间、提取温度、pH和料液比对胶原蛋白提取率的影响,并用正交试验法优化酶法提取工艺。结果表明,40℃条件下水浴循环回流脱脂3 h就可以基本脱除猪跟腱中的脂肪(脱脂率为98.88%);酶法提取最佳工艺条件为:酶用量250 u/g、提取时间25 h、提取温度为25℃、pH1.6、料液比1︰20 g/g,此条件下每百克脱脂猪跟腱可获得45.8 g胶原蛋白;影响酶法提取效果的主次因素排序为:酶用量>提取温度>料液比>提取液pH>提取时间。进一步细化跟腱提取工艺的同时,与其他跟腱工艺比较有缩短了提取时间,节约能源,定量的明确了提取率等优点。

In order to make use of the resource of animal tendons,improve the added value,the collagen is extracted from pig tendons with enzymatic extraction.This research is based on removing fat and the facts of extraction process：enzyme amount（u/g）,time（h）,temperature（℃）,pH and ratio of material to liquid.The extraction rate of the collagen is studied by single factor process.Through the orthogonal experiment,get the optimum condition for extraction.The experimental condition is that enzyme amount is 250 u/g,time is 25 h,temperature is 25 ℃,pH is 1.6,ratio of material to liquid is 1：20 g/g.The yield is 45.8 g collagen per 100 g of defatted pig tendons.In this study,the factors of the process are investigated,and the optimum experimental condition is finally determined.Factors affecting the extraction of sorting are enzymetemperatureratio of material to liquidpHtime.Besides the extraction process of the pigs＇ tendon,there are these advantages which are saving time,saving energy and so on.