鳖甲水解多肽的纯化、鉴定及体外抗肝纤维化活性研究（英文）

Purification,characterization,in vitro anti-hepatic fibrosis activity of bioactive peptides derived from Carapax Trionycis hydrolysates

探讨鳖甲多肽的纯化、鉴定及其体外抗肝纤维化活性。通过超滤、葡聚糖凝胶Sephadex G-15及高效液相色谱法对鳖甲水解产物进行分离,得到一个纯化的寡肽(CTEPH-1:Asn-Pro-Asn-Pro-Thr)。采用MTS法和酶联免疫吸附法测定纯化多肽对活化肝星状细胞的抑制作用,发现通过降低各型胶原蛋白和TIMP-1的含量,CTEPH-1有效的抑制了肝星状细胞的活化和增殖。因此,该寡肽CTEPH-1可以作为功能食品的添加剂或潜在的抗肝纤维化药物。

Anti-hepatic fibrosis peptide from Carapax Trionycis was purified, characterized, and inhibitory effect was assessed. Carapax Trionycis extract peptide hydrolysates （CTEPHs） were separated by ultrafiltration, Sephadex G-15 gel chromatography and RP-HPLC. One novel anti-hepatic fibrosis peptide （CTEPH-I： Asn-Pro-Asn-Pro-Thr） was obtained and identified. MTS assay and enzyme-linked immunosorbent assay were applied to evaluate the anti-fibrotic effect of CTEPH-1 on activated hepatic stellate cells （HSCs） in vitro. CTEPH-1 efficiently inhibited activation and proliferation of cultured HSC-T6 cells via lowering the contents of collagen and TIMP- 1 except for matrix metalloproteinase- 1 （MMP- 1）. The purified peptide might be beneficial as functional food or potential drug for treatment of liver fibrogenesis.