HIF-1α在大鼠造影剂肾病中的表达及对肾小管损伤的影响

Study on Expressions of HIF-1α and Renal Tubular Injury on Contrast-induced Nephropathy of Rats

目的:探讨HIF-1α在造影剂肾病大鼠血清中的表达水平及对肾小管损伤的影响。方法:将45只SD大鼠随机分为三组,每组15只。其中空白对照组(A组)大鼠禁食水12 h后,于尾静脉注射氯化钠注射液0.5 m L,共三次,每次间隔15 min。造影剂肾病组(B组)大鼠禁食水12 h后,于尾静脉以10 mg/kg注射吲哚美辛,15 min后以10 mg/kg注射左旋硝基精氨酸甲酯(L-NAME),15 min后再注射碘比醇(3 g I/kg)。阿托伐他汀钙组(C组)大鼠于实验前3 d开始喂食阿托伐他汀钙片,连续喂食3天,剂量为80mg/kg/d,再禁食水12 h后,制作造影剂肾病模型,步骤同造影剂肾病组。观察并比较三组大鼠肾功能指标(BUN、Cr)变化、HIF-1α表达水平及肾小管损伤情况。结果:造影剂肾病组大鼠BUN水平低于阿托伐他汀钙组和空白对照组,而Scr水平高于阿托伐他汀钙组和空白对照组,差异均具有统计学意义(P<0.05)。阿托伐他汀钙组大鼠BUN水平低于空白对照组,而Scr水平高于空白对照组,差异具有统计学意义(P<0.05)。造影剂肾病组大鼠肾小管损伤高于阿托伐他汀钙组和空白对照组,阿托伐他汀钙组大鼠肾小管损伤高于空白对照组,差异均具有统计学意义(P<0.05)。造影剂肾病组大鼠HIF-1α表达高于阿托伐他汀钙组和空白对照组,阿托伐他汀钙组大鼠HIF-1α表达高于空白对照组,差异均具有统计学意义(P<0.05)。结论:造影剂肾病发生时存在缺氧情况,阿托伐他汀钙在造影剂肾病中具有保护作用。

Objective：To investigate the expression of HIF-1α in serum of rats with contrast induced nephropathy and its effect on renal tubular injury.Methods：45 SD rats were random Ly divided into three groups（n=15）.The rats in the blank control group（group A）were treated with 12 h（Sodium Chloride Injection） for three 0.5 m L after fasting water for a period of about 15 minutes.Contrast nephropathy group（B group） rats after fasting 12 h,in the tail vein with 10 mg/kg injection of indomethacin,15 minutes after the injection of 10 mg/kg nitro-L-arginine methyl ester（L-NAME）,15 minutes after the injection of iobitridol（3 G I/kg）.Atorvastatin group（C group） rats in the first 3 days of the experiment started feeding atorvastatin calcium tablets,continuous feeding for 3 days,at a dose of 80mg/kg/d,and fasting 12 h,making contrast nephropathy model,with the steps of contrast nephropathy group.The changes of renal function indexes（BUN,Cr）,HIF-1α expression and renal tubular injury in three groups were observed and compared.Results：The level of BUN in rats with contrast induced nephropathy was lower than that in atorvastatin calcium group and blank control group,but the level of Scr was higher than that of atorvastatin calcium group and blank control group,the difference was statistically significant（P0.05）.The level of BUN in atorvastatin calcium group was lower than that in blank control group,but Scr level was higher than that in blank control group,the difference was statistically significant（P0.05）.Compared with the blank control group,the renal tubular injury in the rats with contrast induced nephropathy group was higher than that in atorvastatin calcium group and blank control group,the difference was statistically significant（P0.05）.Compared with the control group,the expression of HIF-1 was significantly higher in rats with contrast induced nephropathy than that in atorvastatin calcium group and blank control group.The expression of HIF-1 was significantly higher than that in the control group（P0.05）.Conclusions：It is suggested that the statins could prevent the contrast-induced nephropathy.However,the ending mechanism of statins should be further studied in the clinical practices.