ING4和HIF-1α在口腔鳞癌中的表达及临床意义

Expressions and Clinical Significances of ING4 and HIF-1α in the Oral Squamous Cell Carcinoma

目的:探讨人口腔鳞状细胞癌(OSCC)组织中生长抑制因子4(ING4)和缺氧诱导因子-1α(HIF-1α)的表达及其临床意义。方法:选择我院确诊的口腔鳞癌患者共计65例,均经过手术治疗,术前未行放化疗,切除标本经过石蜡包埋切片,同时选取20例正常口腔黏膜作为对照。采用免疫组化S-P法检测人口腔鳞状细胞癌和正常口腔黏膜组织中ING4和HIF-1α的表达,并分析其与OSCC患者临床病理特征的相关性及人口腔鳞状细胞癌组织中ING4和HIF-1α表达的相关性。结果:OSCC组织中ING4的阳性表达率为41.5%(27/65),显著低于正常口腔黏膜组织(P<0.05);OSCC组织中HIF-1α的阳性表达率为64.6%(42/65),显著高于正常口腔黏膜组织(P<0.05)。ING4和HIF-1α的表达与OSCC的病理分级、TNM分期和是否有淋巴结转移显著相关(P<0.05);OSCC组织中,ING4的表达与HIF-1α的表达呈显著负相关(P<0.05)。结论:口腔鳞状细胞癌组织中ING4的表达下调,HIF-1α的表达上调,二者可能通过负向调控作用在口腔鳞状细胞癌的发生、发展、侵袭和转移中发挥着重要作用。

Objective： To investigate the expressions and clinical significances of ING4 and H1F-1α in the oral squamous cell carcinoma tissues. Methods： Paraffin embedded specimens of 20 cases of normal oral tissues and 65 cases of OSCC tissues were randomly obtained with informed consent from patients who underwent surgery without radical resections in our hospital. The expressions of ING4 and HIF-1α in normal tissues and OSCC tissues were detected by S-P method. The relationship between their expressions and clinicopathologic features of OSCC patients were analyzed. Results： The positive rate oflNG4 in OSCC tissues （41.5%） was significantly lower than that of the normal oral mucosa （P〈0.05）. The positive rate of HIF-1α in OSCC tissues （64.6%） was significantly higher than that of the normal oral mucosa （P〈0.05）. The positive expression rates of ING4 and HIF-1α were significantly correlated with the pathological grade, TNM stage and lymph node metastasis of OSCC （P〈0.05）. There were negative correlation between ING4 and HIF-1α expressions in the OSCC tissues （P〈0.05）. Conclusion： ING4 was downregulated and HIF-1α was upregulated in the OSCCs, which might play an important role in the occurrence, development, invasion and metastasis of OSCC via negative interaction.