超声导入复方三七消痛软膏对兔膝骨性关节炎滑膜形态和软骨细胞caspase-9、XIAP表达的影响

Influences of sonophoresis of Fufang Sanqi Xiaotong(Compound Notoginseng Pain-relieving) Ointment on synovial morphology and expressions of chondrocyte caspase-9 and XIAP in rabbits with knee osteoarthritis

目的观察超声导入复方三七消痛软膏对兔膝骨性关节炎(KOA)滑膜形态和软骨细胞含半胱氨酸的天冬氨酸蛋白水解酶-9(caspase-9)及X-连锁凋亡抑制蛋白(XIAP)表达的影响,探讨超声导入复方三七消痛软膏对KOA的疗效和机制。方法 32只6月龄新西兰白兔随机分为超声导入复方三七消痛软膏治疗组、普通耦合剂超声治疗组、模型组、正常组4组,每组各8只。除正常组外,其余动物均行前交叉韧带切断术(ACLT),术后6周后对超声导入复方三七消痛软膏治疗组、普通耦合剂超声治疗组2组分别用超声导入复方三七消痛软膏和单纯超声治疗2个疗程;模型组和正常组不予干预。2个疗程结束后处死各组动物,HE染色光镜下观察滑膜组织形态学,免疫组化检测各组软骨组织中caspase-9及XIAP的表达情况。结果光镜下滑膜退变程度由轻至重:正常组,超声导入复方三七消痛软膏治疗组,普通耦合剂超声治疗组,模型组。与正常组相比较,超声导入复方三七消痛软膏治疗组、普通耦合剂超声治疗组、模型组caspase-9免疫组化IOD值高于正常组,而XIAP免疫组化IOD值低于正常组;与模型组相比较,超声导入复方三七消痛软膏治疗组、普通耦合剂超声治疗组caspase-9免疫组化IOD值低于模型组,而XIAP免疫组化IOD值高于模型组。超声导入复方三七消痛软膏治疗组caspase-9免疫组化IOD值低于普通耦合剂超声治疗组,而XIAP免疫组化IOD值高于普通耦合剂超声治疗组。结论超声导入复方三七消痛软膏治疗兔KOA优于超声波,能够起到保护软骨和减轻滑膜炎症的作用,延缓KOA的发展,其治疗机制可能是通过降低软骨细胞caspase-9及增加XIAP的表达实现。

Objective To observe the influences of sonophoresis of Fufang Sanqi Xiaotong（ Compound Notoginseng Pain-relieving） Ointment on changes of synovial morphology and expressions of chondrocyte caspase-9 and X-linked inhibitor of apoptosis protein（ XIAP） in rabbits with knee osteoarthritis（ KOA）.Methods New Zealand white rabbits（ n = 32,6 months old） were randomly divided into group of sonophoresis of Fufang Sanqi Xiaotong（ sonophoresis group）, group of coupling agent ultrasound treatment（ coupling agent group）,model group and normal group（ each n = 8）. Except of normal group,other groups received anterior cruciate ligament transaction（ ACLT）. After 6 weeks,sonophoresis group and coupling agent group were given corresponding therapies for 2 treatment courses and model group and normal group were not given any interventions. The changes of synovial histomorphology were observed with light microscope after HE staining,and expressions of caspase-9 and XIAP were detected by using immunohistochemistry technique in all groups after 2 treatment courses. Results The degeneration degree of synovial membrane under light microscope showed an ascending order in all groups as follows： normal group,sonophoresis group,coupling agent group and model group. The value of integral optical density（ IOD） of caspase-9 was higher,and IOD value of XIAP was lower in sonophoresis group,coupling agent group and model group than those in normal group. The IOD value of caspase-9was lower,and IOD value of XIAP was higher in sonophoresis group and coupling agent group than those in model group. The IOD value of caspase-9 was lower, and IOD value of XIAP was higher in sonophoresis group than those in coupling agent group. Conclusion The sonophoresis of Fufang Sanqi Xiaotong is superior to ultrasound treatment,and has the protective effect on cartilage and relief effect on synovial inflammation,and can delay KOA development. The mechanism may be related to decrease of caspase-9 expression and increase of XIAP expression.