蒙药复方述达格-4的大鼠血清化学成分分析

Chemical compositions of Mongolian medicine compound formula-Shudage-4 in rat serum

目的建立蒙药复方述达格-4体内外特征图谱,归属并鉴定其体外化学成分及体内吸收入血成分。方法取Wistar大鼠,灌胃蒙药复方述达格-4及其各单味药有效组分,分离制备含药血清,HPLC法测定,建立蒙药复方述达格-4体外及含药血清HPLC特征图谱,分析蒙药复方述达格-4各单味药材体外及其大鼠含药血清特征图谱,比对、归属并鉴定蒙药复方述达格-4体外化学成分及口服蒙药复方述达格-4后的大鼠吸收入血成分。结果分析蒙药复方述达格-4体外HPLC特征图谱,共发现了64个峰。蒙药复方述达格-4灌胃给药后出现了23个入血成分,17个为体外成分直接入血,另外6个推测为新生代谢产物。蒙药复方述达格-4灌胃给药后出现的23个入血成分全部来源于各单味药材及全方,其中1,2,3,9,23号峰来源于高良姜;14号峰来源于木香;13号峰来源于石菖蒲;15,20,21,22号峰来源复杂,均来源于高良姜、木香、石菖蒲;6,10,17,18,19号峰来源于高良姜、石菖蒲;7,16号峰来源于高良姜、木香;11号峰来源木香、石菖蒲;4,5,8,12号峰来源于全方,并从中鉴定出7个蒙药复方述达格-4成分。结论血中成分及代谢产物可能为蒙药复方述达格-4体内直接作用的有效成分,主要来源于蒙药复方述达格-4及其各单味药。

Objective To establish in vivo and in vitro the characteristic chromatograms of Mongolian medicine compound formula-Shudage-4,and affiliate and identify in vitro chemical compositions of Shudage-4 and its compositions in vivo absorbed into blood. Methods Wistar rats were intragastrically given Shudage-4 and its different single medicinal,and then serum samples were prepared and determined by using high performance liquid chromatography（ HPLC）. The HPLC characteristic chromatograms of in vitro samples of Shudage-4 and its different single medicinal and Shudage-4 medicated serum were established and analyzed. The chemical compositions in vitro of Shudage-4 and its compositions in vivo absorbed into blood after taken by rats were compared,affiliated and identified. Results There were totally 64 peaks found after analyzing in vitro HPLC characteristic chromatograms of Shudage-4. There were 23 chemical compositions absorbed into blood,and 17 in vitro ones absorbed directly into blood and 6speculated as new metabolites. These 23 chemical compositions were all derived from different were derived from Gaoliangjiang（ Rhizoma Alpiniae Officinarum,Galangal Rhizome）,peak 14,from Muxiang（ Radix Aucklandiae,Common Aucklandia Root） and peak 13,from Shichangpu（ Rhizoma Acori Tatarinowii,Grassleaf Sweetflag Rhizome）. The peaks 15,20,21 and 22 were derived from Gaoliangjiang,Muxiang and Shichangpu,peaks 6,10,17,18 and 19,from Gaoliangjiang and Shichangpu,peaks 7 and 16,from Gaoliangjiang and Muxiang,peak 11,from Muxiang and Shichangpu,and peaks 4,5,8 and 12,from the whole formula of Shudage-4,from which 7 chemical compositions were identified. Conclusion The chemical compositions and metabolites in blood may be the active principles of Shudage-4 with direct effects in vitro,which are derived respectively from different single medicinal in Shudage-4.