无硫复合护色剂处理的干制苹果片中差异蛋白表达的分析

Analysis of Differential Protein Expression in Dried Apple Slices Treated with Sulfur Free Composite Color Protection Agent

[目的]分析经无硫复合护色剂处理的苹果片在干制后差异蛋白的表达情况。[方法]采用无标记(Label-free)定量蛋白质组学技术对复合护色剂处理的干制苹果片进行蛋白质组学研究,探讨蛋白质组表达水平的差异。所提取的蛋白质样品经酶解、色谱质谱检测分析,用Label-free定性定量软件maxquant(1.5.0.12)结合搜库定性软件Andromeda,对每个样本进行定性定量,通过蛋白质相对定量的比较寻找差异表达蛋白。[结果]与非处理苹果片相比,差异蛋白总数为909个,上调差异蛋白质319个,下调差异蛋白质590个;生物学过程主要集中在代谢过程、细胞过程。分子功能主要在绑定、催化活性、能量代谢等部分。细胞组件主要集中在细胞膜相关的部件。[结论]该研究可为解析复合护色剂处理后干制苹果片中次生代谢物差异的成因及其抑制褐变的蛋白质机制提供参考。

[Objective] To analyze the expression of differentially expressed proteins in dried apple slices treated with sulfur free composite color protection agent.[Method] Proteomic analysis of dry apple slices treated with composite color protection agents was carried out using unlabeled （Label-free） quantitative proteomic techniques to investigate the differences in protein expression levels.The analysis of the extracted protein samples by enzymolysis and GC-MS detection,using Label-free quantitative software maxquant （1.5.0.12） combined with qualitative database search software Andromeda,the qualitative and quantitative of each sample,the protein relative quantitative comparison for different expression of protein.[Result] Compared with untreated apple slices,the total number of differentially expressed proteins was 909,up to 319 differentially ex-pressed proteins and 590 down regulated proteins.The biological process mainly focused on the metabolic process and the cellular process.The molecular functions were mainly binding,catalytic activity,energy metabolism and so on.Cell components were mainly concentrated in cell mem-brane related components.[ Conclusion] This study can provide a reference for the analysis of the causes of the secondary metabolite differences in the dried apple slices and the mechanism of protein inhibition of browning.