UPLC法测定整参·须根和芦头中人参皂苷的含量

Determination of Ginsenosides in Whole Ginseng,Fibrous Root and Root Refs by UPLC

[目的]建立适用于整参、须根、芦头中人参皂苷的超高效液相色谱(UPLC)检测方法,同时测定人参皂苷Rg_1、Re、Rf、Rg_2、Rb_1、Rc、Rb_2、Rd的含量。[方法]采用ACQUITY UPLC BEH C_(18)色谱柱(1.7μm,2.1 mm×50 mm)分离,流动相为乙腈-0.1 mol/L磷酸溶液,梯度洗脱,检测波长为203 nm,柱温35℃,流速0.3 m L/min。[结果]人参皂苷Rg_1、Re、Rf、Rg_2、Rb_1、RC、Rb_2、Rd线性关系良好,回收率在87.3%~98.6%。[结论]该方法准确、重复性好,适用于整参、须根、芦头中人参皂苷的定量分析。

[Objective] The research aimed to establish a chromatographic separation method for the determination of ginsenosides in the whole ginseng, fibrous root and root refs.Ginsenosides Rg1,Re,Rf,Rg2,Rb1,Rc,Rb2 and Rd were determined.[Method]The separation was performed by a ACQUITY UPLC BEH C18 column （1.7 μm, 2.1 mm ×50 mm）, the mobile phase was acetonitrile -0.1%phosphoric acid aqueous so-lution with gradient elution at a flow rate of 0.3 mL/min, the detection wavelength was set at 203 nm, and the column temperature was 35 ℃. [Result]The method showed a good linear relationship of ginsenosides Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rd.Recovery rate was from 87.3%to 98.6%.[Conclusion]This method is accurate and good reproducibility,and suitable for the determination of ginsenosides in the whole ginseng, fibrous root and root refs.