间接阻抗法检测酸性罐藏食品商业无菌的研究

Study on Detection of Commercial Sterility in Acid Canned Food by Indirect Impedance Method

建立间接阻抗法快速检测酸性罐藏食品商业无菌的方法。选取酸性罐藏食品中易污染的多粘芽孢杆菌、肠膜明串珠菌和异常汉逊酵母作为试验菌株,对间接阻抗法的临界值、适用性、灵敏度、测量持续时间及罐头样品取样量等应用条件进行研究,并与国标法GB 4789.26-2013《食品安全国家标准食品微生物学检验商业无菌检验》进行比对。研究表明,建立的间接阻抗法与国标法的检测结果完全一致,但比常规方法缩短7 d,3 d内即可判定样品是否为商业无菌。

A method for rapid detection of commercial sterility in acid canned food was established by indirect impedance method. The microorganisms which are easily contaminated in acid canned foods, such as Paeni-bacillus polymyxa, Leuconostoc mesenteroides and Hansenula anomala were selected as experimental strains to study of the threshold, applicability, sensitivity, measurement duration and sample volume of canned food of the indirect impedance method. Compared with the national standard method GB 4789.26-2013〈National food safety standard Food microbiological examination：Examination of commercial sterility〈, the study showed that the testing results of the indirect impedance method were completely consistent with that of national standard method. Meanwhile, the detection time was shorter than that of conventional method of 7 days. The sample could be determined whether is commercial sterility within 3 days.