摘要
比较mini-VIDAS、膜芯片两种快速筛检方法和常规培养法,以确定两种快速筛检方法对大肠埃希氏菌O157:H7筛检的效果。以大肠埃希氏菌O157:H7为目标菌,针对3种方法设计灵敏性、特异性、抗干扰性试验和人工污染试验。结果表明,常规培养法和mini-VIDAS法的灵敏性较高,mini-VIDAS法和膜芯片法的特异性和抗干扰性较高,人工污染试验三者表现符合预期。日常检验中可采用国标法和mini-VIDAS法进行筛检工作,应急检验可使用膜芯片法缩短筛检时间,mini-VIDAS法和膜芯片法可作为初步筛检的工具使用。
This study aimed to compare of two rapid pre-detection methods and traditional culture method for detection of Escherichia coli O157：H7. Experiments were designed to test the sensitivity, specificity and anti interference of these methods, artificially contaminated samples were tested and verified. The results showed that, traditional culture method and mini-VIDAS method with high sensitivity, mini-VIDAS and membrane-based array method with specificity and high anti-interference, artificially contaminate test was as expected. Traditional culture method and mini-VIDAS method can be used for pre-detection in daily inspections,mem-brane-based array method could shorten the pre-detection time in emergency testing. Additionally, mini-VI-DAS and membrane-based array are good tools for pre-detection of the Escherichia coli O157：H7.
出处
《食品研究与开发》
CAS
北大核心
2017年第19期112-115,共4页
Food Research and Development
基金
重庆市社会民生科技创新专项项目(cstc2015shmszx00002)
重庆市科技计划项目(cstc2015jcsf8001)
