酶法降解米糠中植酸的工艺研究

Process of Enzymatic Degradation of Phytic Acid in Rice Bran

通过酶法降解米糠中植酸,植酸酶能将植酸及其二价盐类菲丁水解生成肌醇。以酶用量、酶解时间、p H值、酶解温度为影响因素,以代谢产物肌醇含量为判定依据进行单因素试验及正交试验以得出较优组合。通过试验得出四因素对植酸酶酶解植酸所得的肌醇含量的影响大小顺序为:酶用量>p H值>反应时间>反应温度,植酸最佳酶解条件为酶用量60 U/g,反应时间20 min,p H值4.0,反应温度55℃。验证试验测得肌醇含量为66.21 mg。酶解法反应条件温和、耗能低,为工业化生产提供了一种新的思路。

Degradation of phytic acid in rice bran by enzymatic method, phytase can form phytic acid and its divalent salt phenanthroline to produce inositol.The effects of enzyme dosage, enzymolysis time, pH value and en- zymolysis temperature were studied , single factor test and orthogonal test were used to determine the optimal combination of the inositol content of the metabolites. The results showed that the effect of four factors on the phytase content of phytase was enzyme dosage〉 pH〉 reaction time〉 reaction temperature, the phytase optimum conditions for the amount of enzyme 60 U/g, reaction time 20min, pH value of 4.0, reaction temperature 55℃. Verifying test the inositol content was 66.21 mg. Enzymatic hydrolysis has mild reaction conditions, low energy consumption, for the industrial production provides a new idea.