HPLC同时测定加味香薷口服液中6种主要活性成分的含量

Simultaneous Determination of Six Main Active Ingredients in Jiawei Xiangru Oral Liquid by HPLC

目的:建立同时测定加味香薷口服液(葛根、香薷、厚朴、陈皮、蚕沙、白扁豆)中葛根素、麝香草酚、香荆芥酚、和厚朴酚、橙皮苷、大豆苷含量的方法。方法:采用高效液相色谱法。采用Hypersil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.01%冰乙酸溶液(梯度洗脱),流速1 m L·min^(-1),检测波长277 nm,柱温30℃。结果:葛根素、麝香草酚、香荆芥酚、和厚朴酚、橙皮苷、大豆苷分别在0.384~4.80μg(r=0.999 3),0.048~0.60μg(r=0.999 7),0.015 6~0.195μg(r=0.999 8),0.018 2~0.227 5μg(r=0.999 2),0.226~2.825μg(r=0.999 6),0.228~2.850μg(r=0.999 5)进样量与峰面积呈良好的线性关系;平均加样回收率分别是葛根素97.98%,RSD 2.1%,麝香草酚98.88%,RSD 1.8%,香荆芥酚98.10%,RSD 2.1%,和厚朴酚100.12%,RSD 1.5%,橙皮苷100.40%,RSD 2.6%,大豆苷96.70%,RSD 1.6%。结论:该方法操作简便、稳定、重复性好,可用于加味香薷口服液中葛根素、麝香草酚、香荆芥酚、和厚朴酚、橙皮苷、大豆苷含量的同时测定。

Objective： To establish a method for simultaneously determining the contents of puerarin,thymol,carvacrol,honokiol,hesperidin and daidzin in Jiawei Xiangru oral liquid（ Puerariae Lobatae Radix,Moslae Herba,Magnoliae Officinalis Cortex,Citri Reticulatae Pericarpium,Bombyx Mori Linnacus and Lablab Semen Alabum）. Method： High performance liquid chromatography（ HPLC） was performed on Hypersil C18 chromatographic column（ 4. 6 mm × 250 mm,5 μm） with mobile phase of acetonitrile-0. 01% glacial acetic acid（ gradient elution） at a flow rate of 1 m L·min^（-1）. The detction wavelength was 277 nm,and the column temperature was 30 ℃. Result： Puerarin,thymol,carvacrol,honokiol,hesperidin and daidzin showed good linear relationship between injection volume and peak area within the range of 0. 384-4. 800 μg（ r = 0. 999 3）,0. 048-0. 600 μg（ r =0. 999 7）,0. 015 6-0. 195 0 μg（ r = 0. 999 8）,0. 018 2-0. 227 5 μg（ r = 0. 999 2）,0. 226-2. 825 μg（ r =0. 999 6）,and 0. 228-2. 850 μg（ r = 0. 999 5）,respectively. The average recovery rate was 97. 98%,98. 88%,98. 10%,100. 12%,100. 40% and 96. 70%,respectively for puerarin,thymol,carvacrol,honokiol,hesperidin and daidzin,with RSDs of 2. 1%,1. 8%,2. 1%,1. 5%,2. 6%,and 1. 6%. Conclusion： The method is simple,stable and reproducible,and it can be used for the simultaneous determination of puerarin,thymol,carvacrol,honokiol,hesperidin and daidzin contents in Jiawei Xiangru oral liquid.