摘要
目的:探讨脾虚型功能性消化不良(FD)大鼠肝组织细胞色素C氧化酶IV(COX IV)蛋白与基因表达及脾虚1号方干预。方法:70只10 d龄雄性SD大鼠按随机数字表法分为正常对照组(10只)、FD模型组(单模型组10只)、脾虚型FD模型组(50只)。正常组给予2%蔗糖溶液灌胃,FD模型组和脾虚型FD模型组均给予0.1%蔗糖碘乙酰胺蔗糖溶液灌胃,0.2 m L/只·d,连续6 d。脾虚型FD模型组正常饲料喂养至6周龄后叠加改良小平台站立,连续14 d;造模结束后按随机数字表法分为双模型组、多潘立酮组、脾虚1号方低、中、高剂量组各10只,药物干预14 d,采用免疫组化、Western-blot和RT-PCR方法检测肝脏组织COX IV蛋白与mRNA表达量。结果:与正常组比较,双模型组大鼠肝脏COX IV mRNA表达量和平均光密度值显著降低;与双模型组比较,脾虚1号方高剂量组大鼠肝脏COX IV mRNA和蛋白表达量升高。结论:脾虚型FD大鼠存在COX IV mRNA和蛋白表达量降低现象,脾虚1号方可能是通过上调COX IV蛋白与mRNA的表达提高脾虚证大鼠能量代谢。
Objective: To investigate the expression of COX IV protein and mRNA in Liver tissue of Dyspepsia rats with spleen deficiency type and the intervention by Pixu I Recipe. Method: 70 10-day-old male SD rat pups were randomly divided into normal group( n = 10),FD model group( single model,n = 10),FD with spleen deficiency model group( n =50). The normal control group were gavaged with 0. 2 m L of 2% sucrose,meanwhile,the FD with spleen deficiency model group were gavaged with 0. 2 m L of 0. 1% IA in 2% sucrose,for 6 days. To postnatal 43 day,the FD with spleen deficiency model group was given modified multiple platform method( MMPM),continued 14 h every day,for 14 days. The FD with spleen deficiency model group were averagely randomly divided into double model group,domperidone group,low-dose,medium-dose and high-dose Chinese herbs group,and treated by drug for 14 d. To detect the COX IV protein expression in liver tissues by immunohistochemistry,Western-blot and RT-PCR. Results: Compared with the normal group,the mRNA and protein expression of COX IV of double model group were significantly decreased; Compared with the double model group,the mRNA and protein expression of COX IV of high-dose Pixu I Recipe group were increased. Conclusion: The FD Rats with Spleen Deficiency had the phenomenon of low expression of COX IV mRNA and protein,and Pixu I Recipe could increase the expression of COX IV protein and mRNA,and then to promote metabolic energy.
出处
《中国中医基础医学杂志》
CSCD
北大核心
2017年第9期1234-1237,1252,共5页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家重点基础研究发展计划(2013CB531703)-"脾失健运"所致功能性胃肠病"从脾论治"疗效及机制研究
国家自然科学基金青年基金项目(81503567)-基于STIM/TRPC/SOCC通路探讨脾虚型FD大鼠胃动力障碍及香砂六君子汤干预机制
中国博士后科学基金面上项目(2015M1227)-基于IP3/Ca2+通路探讨香砂六君子汤干预脾虚型FD大鼠作用机制
中国博士后科学基金特别资助项目(2016T90195)-基于H+/PAR2/TRPV1通路探讨香砂六君子干预FD大鼠机制
关键词
脾虚证
功能性消化不良
细胞色素C氧化酶
中医
Spleen deficiency syndrome
Functional dyspepsia
Cytochrome c oxidase
Traditional Chinese medicine