辣根过氧化物酶催化的透明质酸水凝胶的制备及表征

Preparation and characterization of horseradish peroxidase catalyzed hyaluronic acid hydrogel

目的制备辣根过氧化物酶催化交联的透明质酸水凝胶,并考察其性能。方法透明质酸经酪胺修饰后,在辣根过氧化物酶及H_2O_2催化下形成透明质酸水凝胶,考察处方中辣根过氧化物酶及H_2O_2对凝胶形成时间、溶胀率、水凝胶微观形态及其体外降解特性的影响。结果辣根过氧化物酶能在H_2O_2存在的条件下成功催化了透明质酸-酪胺之间分子交联,形成水凝胶,且在H_2O_2浓度低、辣根过氧化物酶浓度高时,水凝胶内部的交联程度、溶胀率更高,在体外降解所需的时间也越长。结论采用辣根过氧化物酶催化交联的方式能成功制备透明质酸水凝胶。

OBJECTIVE To prepare horseradish peroxidase catalyzed hyaluronic acid hydrogel and to investigate the influence of HRP and H2O2 on hyaluronic acid hydrogel. METHODS Hyaluronic acid was conjugated with tyramine and catalyzed by horseradish peroxidase and H2O2 to form hyaluronic acid hydrogel. The influence of horseradish peroxidase and H2O2 on hydrogel gelatin time,swelling ratio,morphology and in vitro degradation were also evaluated. RESULTS Hyaluronic acid-tyramine could be successfully catalyzed by horseradish peroxidase and forms hydrogel. With the decrease in H2O2 concentration or increase in horseradish peroxidase concentration,the crosslinking degree of hyaluronic acid hydrogel was increased,the swelling ratio was increased,and the time for completely degradation of hyaluronic acid hydrogel was also increased. CONCLUSION Horseradish peroxidase catalyzed crosslinking method can successfully lead to the formation of hyaluronic acid hydrogel.