HPLC同时测定香砂平胃颗粒中的橙皮苷、和厚朴酚与厚朴酚

Simultaneous determination of hesperidin,honokiol and magnolol in Xiangsha Pingwei granules by HPLC

目的测定香砂平胃颗粒中3种有效成分的含量。方法采用HPLC法测定橙皮苷、和厚朴酚、厚朴酚,采用SERVO PT-C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇(A)-水(B),梯度洗脱(0~17 min、37%A,17~17.1 min、37%A^80%A,17.1~29 min、80%A),检测波长284 nm,流速1.0 m L·min^(-1),柱温35℃,进样量10μL。结果 133.42~2134.72 ng橙皮苷、10.98~175.68 ng和厚朴酚、13.95~223.20 ng厚朴酚与峰面积呈良好的线性关系(r分别为0.9990、0.9992、0.9993),平均加样回收率分别为98.4%(RSD=1.87%)、95.7%(RSD=1.96%)、97.3%(RSD=1.35%)。结论所用方法简便可行、重复性好,可用于测定香砂平胃颗粒中各有效成分的含量。

OBJECTIVE To simultaneouly determine three effective components in Xiangsha Pingwei granules（ XPG）. METHODS The contents of hesperidin,honokiol and magnolol were determined by HPLC. The chromatographic conditions were as follows： The SERVO PT-C（18） column（ 250 mm × 4. 6 mm,5 μm） was used as chromatographic column,methanol（ A）-water（ B） as mobile phase,gradient elution： adopted 0-17 min,37% A,17-17. 1 min,37% A-80% A,17. 1-29 min,80% A,with detecting wavelength at284 nm,the column temperature of 35 ℃ and flow rate of 1. 0 m L·min^（-1）. RESULTS A good linearity of hesperidin,honokiol and magnolol was in the range of 133. 42-2134. 72 ng（ r = 0. 9990）,10. 98-175. 68 ng（ r = 0. 9992）,13. 95-223. 20 ng（ r = 0. 9993）,respectively. The average recoveries were 98. 4%（ RSD = 1. 87%） for hesperidin,95. 7%（ RSD = 1. 96%） for honokiol and 97. 3%（ RSD = 1. 35%） for magnolol. CONCLUSION The method is simple,feasible and repeatable,and can be used for the quality control of XPG.