破壁方式对蜂花粉抑制α-葡萄糖苷酶活性的影响

Effects of wall breaking manners on the inhibition of alpha glucosidase activity in bee pollen

以不同破壁方式处理过的油菜蜂花粉为研究对象,提取水溶活性成分,以4-硝基苯-α-D-吡喃葡萄糖苷(PNPG)作为底物,对蜂花粉作为天然α-葡萄糖苷酶活性抑制剂的功效进行了评价。结果表明:破壁方式对蜂花粉功能活性成分以及功效评价结果影响显著,温差破壁、超临界二氧化碳破壁、超声辅助酶解破壁及对照组对α-葡萄糖苷酶的抑制率分别为(13.73±1.15)%,(-8.07±0.93)%,(16.70±0.87)%,(9.68±0.89)%;油菜蜂花粉多糖对α-葡萄糖苷酶的抑制率在(3.46±0.31)%^(-0.78±0.03)%,无明显线性关系;破壁后的样品多糖含量显著增加,但油菜蜂花粉多糖对α-葡萄糖苷酶活性无作用,可能与黄酮等其它成分相关。

The wall of rape bee pollens were broken by using different methods, and them the water-soluble ingredients were extracted. Therefore, the 4-Nitrophenyl a-D-glucopyranoside （PNPG） was as substrate to evaluate the efficiency of bee pollen as a natural alpha glueosidase inhibitor. The results showed that the wall-breaking manners had significant effect on the active ingredient. The inhibition rate of thealphaglucosidase was found ordered as following, tempera- ture difference （ 13.73±01.15）%, supercritical CO2（-8.07±0.93）%, ultrasonic assisted enzymatic （16. 70±0. 87 ）%, and untreated （9.68±0.89）%. Moreover, the inhibition rate of rape pollen polysaccharide on alpha glucosidase activity was fluctuated at （2.46±0.31）%（-0.78±0.03）%, and there was no obvious linear relationship. Finally, after the walls were broken, the content of polysaccharides increased significantly, but rape pollen polysaeeharide had no effect on alpha glucosidase activity. This might be related to flavonoids or other components.