磁化水培养对大球盖菇菌丝生长特性的影响

Effect of magnetized water liquid cultivation on growth characteristics of Stropharia rugoso-annulata mycelium

采用磁化水液体培养大球盖菇菌丝,通过测定菌丝生物量、生长速度、营养成分含量和酶活性,分析磁化水液体培养对大球盖菇菌丝生长规律的影响。结果表明:磁化水培养可以显著提高菌丝生长速度、营养物质含量和酶活性(p<0.05),且最适磁场强度为0.3 T。当磁场强度为0.3 T时,生长稳定期提前36 h,大球盖菇菌丝生长速度提高27.64%,生物量提高27.44%,可溶性蛋白含量提高1.34%,多糖含量提高28.08%,纤维素含量提高34.74%,蛋白酶活性提高14.08%,淀粉酶活性提高21.42%,羧甲基纤维素酶活性提高17.13%,漆酶活性提高19.62%,多酚氧化酶活性提高19.33%。说明可以利用磁化水液体培养显著缩短大球盖菇原种制种周期、提高菌种产量和质量。

The dry weight,growth rate,nutrient content and enzyme activity of Stropharia rugoso-annulata mycelium were determined to reveal effect of magnetized water liquid fermentation on growth characteristics of Stropharia rugoso-annulata mycelium.Results showed that magnetized water culture could improve the dry weight,growth rate,nutrient content and enzyme activity of Stropharia rugoso-annulata mycelium.And the optimum magnetic field strength was 0.3 T which growth stable period was advanced 36 h,growth rate and biomass was improved by 27.64%,and 27.44% respectively,contents of soluble proteins,polysaccharides and cellulose was improved 1.34%,28.08%,and 34.74% respectively,activities of protease,amylase,carboxymethyl cellulose,laccase and polyphenol oxidase were increased by 14.08%,21.42%,17.13%,19.62%,and 19.33%respectively.Magnetic water could shorten the culture period,improving the yield and quality of Stropharia rugoso-annulata strains significantly.