HPLC同时测定复方鱼腥草片中4个有效成分的含量

Simultaneous Determination of 4 Effective Components in Fufang Yuxingcao Tablets by HPLC

目的建立HPLC同时测定复方鱼腥草片中绿原酸、连翘酯苷A、槲皮苷和黄芩苷含量的方法。方法采用CAPCELL PAK MG(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈(A)-0.1%磷酸溶液(B),梯度洗脱(0~12 min,9%A;12~15 min,9%→15%A;15~32 min,15%A;32~48 min,15%→29%A;48~60 min,29%A),流速1.0 m L·min^(-1),柱温35℃,检测波长:330 nm。结果绿原酸、槲皮苷浓度在2~100μg·m L-1内,连翘酯苷A浓度在4~200μg·mL^(-1)内,黄芩苷浓度在12~600μg·m L-1内,与峰面积均呈良好的线性关系(r=0.999 9);平均回收率(n=6)分别为98.0%,99.8%,99.1%和98.3%,RSD均<2.0%。结论建立的HPLC可实现同时测定绿原酸、连翘酯苷A、槲皮苷和黄芩苷4种有效成分,方法快速、简便、结果准确,可为复方鱼腥草片提供更全面、可靠的质量控制方法。

OBJECTIVE To establish an HPLC method for simultaneous determination of chlorogenic acid, forsythoside A, quercitroside and baicalin in Fufang Yuxingcao tablets. METHODS The samples were separated on a CAPCELL PAK MG（4.6 mm×250 mm, 5 μm） column, by a gradient elution using acetonitrile（A） and 0.1% phosphoric acid（B）（0-12 min, 9%A; 12-15 min, 9%→15%A; 15-32 min, 15%A; 32-48 min, 15%→29%A; 48-60 min, 29%A） as mobile phase at a flow rate of 1.0 m L·min^（-1）. The column temperature was set at 35 ℃. The wavelength was set on 330 nm. RESULTS The linear ranges of chlorogenic acid, quercitroside, forsythoside A and baicalin were 2-100 μg·mL^（-1）, 2-100 μg·mL^（-1）, 4-200 μg·mL^（-1） and 12-600 μg·mL^（-1）（r=0.999 9）, the average recoveries（n=6） were 98.0%, 99.8%, 99.1% and 98.3%, respectively, and RSDs were 2.0%. CONCLUSION A rapid, simple, accurate HPLC method is successfully established for simultaneous determine 4 effective components in Fufang Yuxingcao tablets. The method herein described is helpful for the quality control of Fufang Yuxingcao tablets.