沙棘粉对大鼠肝脏脂质代谢及氧化应激的影响

Effect of Sea Buckthorn Powder on Hepatic Lipid Metabolism and Oxidative Stress in Rats

本文研究了沙棘粉对高脂膳食大鼠肝脏脂质代谢及氧化应激的影响。将50只成年雄性Wistar大鼠按体重随机分为对照组、高脂模型组、沙棘低剂量、中剂量和高剂量组5个组。对照组给予基础饲料,其余各组给予高脂饲料饲养,同时每日分别用0.5 mg/g bw、2.5 mg/g bw和5 mg/g bw的沙棘粉匀浆液灌胃大鼠。4 W后处死动物,采集肝脏,分别测定肝脏脂质含量、脂代谢相关酶活性和氧化应激水平。结果表明,与高脂模型组比较,一定剂量的沙棘粉可降低肝脏总胆固醇(TC)和甘油三脂(TG)水平,提高高密度脂蛋白(HDL-C)水平;使肝脏组织肝脂酶(HL)和脂蛋白脂酶(LPL)活性增强;还原型谷胱甘肽(GSH)含量和总超氧化物歧化酶(T-SOD)活力提高,丙二醛(MDA)含量下降。说明沙棘粉可降低高脂膳食大鼠肝脏脂质水平,提高肝脏脂代谢酶活性,增强抗氧化能力,减缓肝细胞的脂质过氧化。

The effects of sea buckthom powder on the hepatic lipid metabolism and oxidative stress in rats were studied in this paper. Fifty male Wistar rats were randomly divided into five groups： the control group; the high fat/cholesterol diet group; and sea buckthom powder low-, medium-, and high-dose groups. The control group was fed with basal diet, and other groups were fed with high fat/cholesterol diet during the experiment. The rats in both control and high fat/cholesterol diet groups were given distilled water, and those in low-, medium-, and high-dose treated groups were given sea buckthorn powder at doses of 0.5 mg/g.body weight （bw）, 2.5 mg/g.bw, and 5 mg/g.bw, respectively. The animals were sacrificed after four weeks, and livers were collected to measure the liver lipid content, lipid metabolism-related enzyme activity, and oxidative stress level. The results indicated that compared with the high fat/cholesterol diet group, sea buckthom powder reduced the levels of hepatic total cholesterol （TC）, total triglyceride （TG）, and malondialdehyde （MDA）, and increase the levels of high density lipoprotein-cholesterol （HDL-C） and glutathione （GSH） and the activities of hepatic lipase （HL）, lipoprotein lipase （LPL）, and total superoxide dismutase （T-SOD）. These results demonstrated that sea buckthorn powder could reduce the liver lipid levels, improve lipid metabolism enzyme activities in the liver, increase antioxidant capacity, and slow down lipid peroxidation in hepatocytes of high-fat diet-fed rats.