明胶-溶菌酶/ZnO纳米复合抗菌膜材料构建与性能

Fabrication and Characterization of Gelatin-Lysozyme/Nano-Zno Nanocomposite Films

本研究以温敏性的明胶为基材同时包埋有机/无机抗菌剂,企图实现抗菌剂的协同增效,扩大材料的抗菌谱,并系统研究抗菌材料表面结构、机械与阻隔性能随抗菌剂种类及计量的演变规律。溶菌酶/纳米氧化锌-明胶膜材料具有选择吸光性,在紫外区域有明显吸收峰,而在可见光区域透光率超过90%。溶菌酶/纳米氧化锌具有协同增效,扩大膜材料的抗菌谱,相对荷载溶菌酶或纳米氧化锌的膜材料,复合抗菌剂膜对大肠杆菌与枯草芽孢杆菌具有更强的抗菌效果。荷载溶菌酶或纳米氧化锌不改变明胶膜材料的表面微结构,两者在成膜及膜基质中的相互作用形成异质的膜表面,并影响蛋白网络结构,弱化膜材料的水汽及氧气阻隔性能。本研究为新型有机/无机复合抗菌膜材料开发提供理论依据。

This work attempted to achieve effective antibacterial gelatin films by incorporating organic / inorganic antibacterials leading to synergistic antibacterial effect and wide antibacterial spectrum. Surface morphology, mechanical and barrier properties as well as antimicrobial activity of the films were evaluated. Gelatin-lysozyme/nano-ZnO nanocomposite films had selective light absorbance. Obvious absorption peak occurred in the ultraviolet （UV） region, while the transmittance was over 90% in the visible region. The simultaneous incorporation of lysozyme / nano-ZnO resulted in synergistic antibacterial activity against test organism, i.e., Escherichia coli （G-） and Bacillus subtilis （G＋）. The loading of lysozyme or nano ZnO within the gelatin matrix did not affect the surface morphology of the films, while the simultaneous loading of lysozyme and nano-ZnO resulted in heterogeneous surface morphology, probably due to the formation of the agglomerates via the interaction between lysozyme and nano-ZnO in the filim-forming solution or the casting process. As a result, gelatin network structure as well as the performance of water vapor and oxygen barrier was varied, in an antimicrobial type-and dose- dependent manner. This study provides a theoretical basis for the development of novel organic/inorganic composite films.