电针“内关”对心肌缺血大鼠相关经穴和非相关经穴皮肤微循环血流灌注量的影响

Effect of Electroacupuncture at “Neiguan”(PC 6)on Skin Microcirculation Blood Perfusion Volume of Heart-related Acupoints and Heart-irrelevant Acupoints in Myocardial Ischemia Rats

目的:探讨大鼠心肌处于正常生理态、缺血损伤病理态时,低频电针和高频电针刺激"内关"后相关经穴和非相关经穴皮肤血流灌注量的变化情况。方法:Wistar大鼠随机分为空白组、假手术组、模型组、低频电针组、高频电针组,每组8只。采用冠状动脉左前降支结扎法复制大鼠急性心肌缺血模型,假手术组只穿线不结扎。低频电针组和高频电针组于造模成功后分别以2Hz及100Hz电针左侧"内关"穴20min,每日治疗1次,于第3次治疗结束后检测各组大鼠心电图J点差值,酶联免疫吸附法检测血清心肌肌钙蛋白T(cTnT)的含量,应用激光散斑衬比成像技术观测各组大鼠双侧"内关""足三里"及"阳陵泉"穴区的皮肤血流灌注量。结果:模型组心电图J点差值及血清中cTnT的含量较空白组及假手术组明显升高(P<0.01);低频电针组、高频电针组电针后心电图J点差值及血清中cTnT的含量较模型组均明显降低(P<0.01,P<0.05)。模型组双侧"内关""足三里"穴区皮肤血流灌注量较空白组显著降低(P<0.01);低频电针组、高频电针组电针后,"内关""足三里"穴区皮肤血流灌注量较模型组均显著升高(P<0.01)。各组"阳陵泉"穴区皮肤血流灌注量无明显变化(P>0.05)。结论:大鼠心肌处于不同状态时,相关经穴穴区皮肤血流灌注量存在一定的变化特征,说明"内关"穴和"足三里"穴区皮肤血流灌注量可以相对特征性地反映心肌状态变化的情况。

Objective To observe the effect of low-frequency or high-frequency electroacupuncture （EA） stimulation of ＂Neiguan＂ （PC 6） on changes of skin blood perfusion volume of heart-related and heart-irrelevant acupoints in rats with normal or ischemic myocardium. Methods Wistar rats were randomly divided into the control, sham-operation, model, low-frequency EA and high-frequency EA groups （n = 8 in each group）. The myocardial ischemic （MI） model was established by occlusion of the anterior descending branch of the left coronary artery. 2 Hz and 100 Hz EA was respectively applied to left PC 6 for 20 min, once daily, a total of three times. Electrocardiogram （ECG） was recorded after the last treatment. Serum cardiac troponin T （cTnT） contents were assayed by ELISA. The skin blood perfusion volumes of bilateral PC 6, ＂Zusanli＂ （ST 36） and ＂Yanglingquan＂ （GB 34） were observed by laser speckle contrast imaging. Results In comparison with the control and sham-operation groups, the differences of ＂J point＂ and contents of serum cTnT were significantly increased in the model group （P〈0.01）. After EA, the differences of ＂J point＂ and contents of serum cTnT were significantly decreased in both low-frequency and high-frequency EA groups （P〈0.01, P〈0.05）. Compared with the control group, the skin blood perfusion volumes of bilateral PC 6 and ST 36 decreased obviously in rats of the model group （P〈0.01）. After EA, the skin blood perfusion volumes were markedly up-regulated in the areas of PC 6 and ST 36 （P〈0.01）. There were no significant changes in that of GB 34 among the 5 groups （P〉0.05）. Conclusion Skin blood perfusion volumes around PC 6 and ST 36 can specifically reflect the change of the myocardial state.