PFKFB3在结肠癌患者中的表达及其临床意义

Expression of PFKFB3 in human colon cancer and its clinical significance

目的:检测6-磷酸果糖激酶-2/果糖双磷酸酶-2同工酶3(PFKFB3)在大样本结肠癌患者中的表达情况及其临床意义。方法:用反转录-聚合酶链反应(RT-PCR)和免疫组化法分别检测156例结肠癌患者癌和癌旁组织中PFKFB3的mRNA和蛋白表达水平,分析其与结肠癌临床病理特征及预后的关系。结果:PFKFB3的mRNA和蛋白表达在114例(73.1%)患者的结肠癌组织中高表达。结肠癌组织中PFKFB3的mRNA水平高表达与脉管内癌栓形成、淋巴结转移和TNM分期相关(P<0.05)。PFKFB3 mRNA高表达患者的生存期短于低表达患者(P<0.05)。结论:PFKFB3在结肠癌患者癌组织中高表达,PFKFB3的mRNA水平高表达与患者恶性表型及不良预后相关。

Objective：To investigate the expression of PFKFB3 in human colon cancer and its clinical significance.Methods：Reverse transcriptase polymerase chain reaction（RT-PCR）and immunohistochemistry were used to detect the mRNA and protein expression levels of PFKFB3 in cancer and adjacent tissues of 156 human colon cancer patients.The relationship of PFKFB3 expression with clinicopathological features and prognosis of colon cancer patients was analyzed by statistical analyses.Results：PFKFB3 mRNA and protein levels were significantly increased in tumor tissues compared to adjacent non-malignant tissues.Furthermore,correlation analysis indicated that PFKFB3 mRNA high expression in colon cancer tissues was significantly associated with intravascular cancer embolus,lymph node metastasis,and TNM staging（P〈0.05）.Survival analysis showed a poor prognosis of patients with high expression of PFKFB3 mRNA level（P〈0.05）.Conclusions：PFKFB3 is highly expressed in colon cancer.The expression level of PFKFB3 is correlated with the malignant phenotype and poor prognosis of colon cancer.