血管紧张素Ⅱ对人子宫内膜上皮细胞增殖和活力的影响

Effect of angiotensinⅡon proliferation and activity of human endometrial epithelium cells

目的观察和分析血管紧张素Ⅱ(AngⅡ)对人子宫内膜上皮细胞(EECs)增殖和活力的影响。方法分离原代EECs并常规培养。试验分为对照组和AngⅡ组。对照组:加入不含血清的培养液;AngⅡ组:加入不同浓度的AngⅡ,通过噻唑蓝(MTT)染色察看各组细胞的增殖率;用ELISA法分析各组孵育液中Ⅰ型胶原(ColⅠ)和纤粘连蛋白(FN)的含量。免疫细胞化学技术分析各组EECs胞质内的α-平滑肌肌动蛋白(α-SMA)和上皮性钙粘附素(E-cadherin)的表达水平。结果不同浓度AngⅡ组的EECs增殖率均显著升高,且表现为剂量依赖的趋势;细胞内α-SMA含量(71.71±1.47)升高,E-cadherin含量(25.32±1.43)降低;ColⅠ和FN分泌量(0.774 7±0.048 0;0.758 0±0.042 0)均增加,与对照组比较,其差异有统计学意义(P<0.05)。结论 AngⅡ能使子宫内膜上皮细胞增殖,并刺激使其发生活力改变。

[Objective] To explore the effect of angiotensin Ⅱ（Ang Ⅱ） on proliferation and activity of human endometrial epithelium cells（EECs）. [Methods]The EECs was isolated and cultured in vitro. There were the control group and Ang Ⅱgroups in the study. EECs in control group were cultivated by solution without serum,and EECs in AngⅡ groups were cultivated by solution with different concentrations of AngⅡ. The method of MTT was used to detect the proliferation of EECs. The contents of Collagen type I（ColⅠ）and Fibronectin（FN）in the cultured supernatants were measured by enzyme-linked immunosorbent assay（ELISA）. The expression levels of α-smooth muscle actin（α-SMA） and Epithelial cadherin（E-cadherin） in cytoplasm of EECs were detected by immunocytochemistry method.[Results]The proliferation rates of EECs in Ang Ⅱ groups significantly increased,and appeared a dose dependent trend. Compared with control group,α-SMA content（71.71 ±1.47） increased and E-cadherin content（25.32±1.43）decreased in AngⅡ groups,the contents of ColⅠ（0.774 7±0.048 0）and FN（ 0.758 0±0.042 0）both increased,and differences were statistically significant（P0.05）.[Conclusion]Ang Ⅱ can promote EECs proliferation,and stimulate EECs to change its vitality.