基于宏基因组学研究中医“肺与大肠相表里”的初步分析与设想

Preliminary analysis and assumption of “the lung and the large intestine being interior-exteriorly related”in Chinese medicine based on metagenomic

在中医学中,"肺与大肠相表里"是重要的脏腑相关理论,自古便有效的指导着中医关于肺肠相关疾病的治疗,受到了学术界的广泛关注。随着现代分子生物学的发展,宏基因组学作为21世纪生命科学的产物,其是以生态环境中的所有细菌与真菌的基因组DNA作为研究对象,通过克隆与异源表达的方式筛选出有用的基因及其产物,并进一步研究这些细菌与真菌的基因组DNA之间的功能与彼此之间的相互作用与联系。因此能够摆脱传统只依靠培养微生物基因的方式来进行研究的束缚,显著扩大微生物基因的可探测范围,近些年来广泛应用于医学研究领域当中。本文通过对肺与大肠相表里的机制及临床研究的情况进行初步的归纳、分析并对宏基因组学技术及其在肺、肠疾病中的研究应用进行介绍,旨在提出借助宏基因组学的技术与方法来研究中医"肺与大肠相表里"理论的初步设想。

＂ The lung and the large intestine being interior-exteriorly related＂ is one of the important theory of viscera correlation in TCM, the theory effective guidance of Chinese medicine in the treatment of pulmonary and intestinal diseases since ancient times, has received extensive attention from the academic circles. With the development of modern molecular biology, Metagenomics as a product of life science in 21st century, the genome of DNA of all bacteria and fungi in the ecological environment is regarded as object of study, through cloning and heterologous expression to screen out the useful genes and their products, further study the function and interaction between the genome DNA of these bacteria and fungi. Therefore, it is possible to get rid of the bondage of traditional methods that only rely on the cultivation of microbial genes, and significantly expand the detection range of microbial genes, which has been widely used in the field of medical research in recent years. This paper summarizes and analysis the mechanism and clinical research of ＂The lung and the large intestine being interior-exteriorly related＂, and metagenomic technology and its application in lung and intestine disease are introduced, to propose a preliminary idea to study ＂The lung and the large intestine being interior-exteriorly related＂ theory with the techniques and methods of metagenomic.