响应面优化超声辅助提取胡萝卜叶总黄酮的工艺研究

Optimization of ultrasound-assisted extraction of total flavonoids from daucus carota leaves using response surface methodology

采用超声辅助提取法对胡萝卜叶中黄酮类化合物进行提取。在单因素试验的基础上,选择超声温度、液料比、乙醇浓度、超声时间为自变量,总黄酮提取率为响应值,研究四个因素及其交互作用对总黄酮提取率的影响。利用响应面进行分析,确定超声辅助提取胡萝卜叶总黄酮的最佳工艺条件为:超声温度为71℃,液料比为15mL/g,乙醇浓度为72%,超声时间为40 min。在上述条件下,总黄酮的提取率达到42.13 mg/g,与预测值的相对误差为0.33%,说明该回归模型的拟合度较高,可用于胡萝卜叶总黄酮提取工艺的优化。

The ultrasonic assisted extraction method was used to extract the total flavonoids from daucus carota leaves. Based on single factor experiments, the total flavonoids extraction yield as the response value. Four influence factors including ultrasonic temperature, liquid-to-material ratio, ethanol concentration and ultrasonic time and their interaction on the total flavonoids were investigated. The optimum process conditions of ultrasonic assisted extraction of total flavonoids from daucus carota leaves was obtained using response surface methodology. The optimal conditions was ： ultrasonic temperature 71 ~C, liquid-to-material ratio 15mL/g, ethanol concentration 72% and ultrasonic time 40 min. The yield of total flavonoids could reach to 42.13 mg/g and the relative error was 0.33% compared to the predicted value. The regression model fitted well with the experimental data. The response surface methodology could be used to optimize the total flavonoids extraction from daucus carota leaves.