超声辅助提取黄皮果核多酚工艺的研究

Ultrasonic-assisted extraction of polyphenols from wampee seeds

天然多酚物质具有各种生物活性而受到广泛关注,从食品加工副产物中回收多酚等活性物质是研究热点。为了从黄皮果核中回收多酚,以黄皮果核为原料,利用超声波技术辅助提取多酚,并采用响应面法优化提取工艺。以多酚提取率为响应值,首先通过单因素试验考察乙醇浓度、超声时间、料液比对提取率的影响,选择因素水平,再通过Box-Behnken试验设计和响应面法优化黄皮果核多酚最佳提取参数。结果表明:乙醇浓度和料液比对提取率的影响显著,超声时间对提取率的影响不显著,料液比的影响最大,而超声时间的影响最小;乙醇浓度与超声时间的交互作用对提取率的影响显著,而乙醇浓度与料液比、超声时间与料液比对的交互作用都对提取率的影响不显著;黄皮果核多酚最佳提取工艺为乙醇浓度52%、超声时间49min、料液比1∶33g/mL,此条件下提取率为7.16mg/g,与预测值偏差0.57%,说明该模型用于提取黄皮果核多酚是可靠的。

Natural polyphenols have attracted much attention because of their biological activities. It is a research hotspot of recovering polyphenols and other active substances from food processing by-products. Wampee seeds were used as the raw material to extract polyphenols by ultrasonic-assisted extraction technology, and response surface methodology was used to optimize the extraction process. With polyphenol extraction yield as response value, the single factor test was first applied to investigate the effect of ethanol concentration, ultrasonic time and solid-liquid ratio on the yield. Then the extraction parameters of polyphenols were optimized by Box Behnken design and response surface methodology. The results showed that the effect of ethanol concentration and solid-liquid ratio were significant on the yield while ultrasonic time was not significant on the yield ~ solid-liquid ratio was the greatest influence factor and ultrasonic time was the least factor. The interaction between ethanol concentration and ultrasonic time had a significant effect, while ethanol concentration and solid-liquid ratio, and ultrasonic time and solid-liquid ratio had little effect. The optimal extraction parameters were .. ethanol concentration of 52%, ultrasonic time of 49min and solid-liquid ratio of 1 ： 33g/mL. Under the above conditions, the yield was 7.16mg/g and the prediction value deviation was 0.57%. These results indicate that the model of extracting polyphenols from wampee seeds is reliable.