新疆地区不同民族丙型肝炎病毒基因分型分布

Distribution of hepatitis C virus genotyping of different nationalities in Xinjiang area

目的了解新疆地区不同民族之间丙型肝炎病毒基因分型的分布特征。方法用RT-PCR方法及PCR-荧光探针法对新疆地区278例丙型肝炎患者进行了HCV RNA的检测和基因分型。结果 278例抗HCV阳性标本中,共检出5种基因型。其中1b型170例(61.15%);2a型77例(27.69%);3a型17例(6.12%);3b型13例(4.68%);6a型1例(0.36%)。不同性别中1b型检出率较高,不同性别间基因型2a和3a检出率差异均有统计学意义(P<0.05),不同性别间基因型1b、3b、6a检出率差异无统计学意义(P>0.05)。1b型和2a型在汉族与少数民族之间分布差异有统计学意义(P<0.05)。3a、3b及6a型在汉族与少数民族之间分布差异无统计学意义(P>0.05)。结论新疆地区HCV基因型以1b型为主,基因型分布呈现多样性。

Objective To understand the distribution of hepatitis C virus genotyping of different nationalities in Xinjiang area. Methods HCV RNA and genotype of 278 patients with hepatitis C virus in Xinjiang area were detected by RT - PCR and PCR -fluorescence probe methods. Results 5 genotypes were detected in 278 anti - HCV positive samples, and 170 cases were infected with genotype lb（61.15% ）, 77 cases were iflfected with genotype 2a（27.69% ）, 17 cases were infected with genotype 3a（6.12% ） , 13 cases were infected with genotype 3b （4.68%） , 1 case was infected with genotype 6a （0.36%）. The detection rate of genotype 1 b was higher than others between men and women. There was statistical significance on the difference in the detection rates of 2a and 3a between different genders（ P 〈 0.05 ）. There was no statistical significance on the difference in the detection rates of 1 b, 3b and 6a between different genders （P 〉 0.05 ）. There was statistical significance on the differences in the detection rates of 1 b and 2a between Han and minority nationalities（ P 〈 0.05 ）. There was no statistical sig- nificance on the differences in the detection rates of 3a, 3b and 6a between Han and minority nationalities（ P 〉 0.05 ）. Conclu- sion Genotype lb was the main type of hepatitis C virus in Xinjiang area, and distribution of genotype is diverse.