Analysis on Genetic Differences between Parents of Two-line Medium Indica Hybrid Rice by SSR Markers

基于SSR标记的中籼型两系杂交水稻亲本遗传差异分析（英文）

The genetic diversity of 50 parents or improved lines of two-line medium Indica hybrid rice were analyzed based on 48 pairs of SSR markers. The results showed that the clustering results were highly accordant with the parental pedigree information of the tested materials. The male sterile lines and the restoring lines were clustered into 2 distinct groups （Ⅰ and Ⅱ）, respectively. There were no significant difference in intra-group average genetic distances （GD） between the 2 groups. Most of the male sterile lines from Hunan Longping High-tech Seed Science Research Institute Co., Ltd. were grouped into a single subgroup （Ⅰ-4） under the male sterile group with the largest average GD compared with the rest male sterile subgroups. Huazhan derived lines and Guangzhou inbred lines were founded to be independent of 9311 and its derivatives or closely-related lines. Meanwhile, they have obviously been differentiated into 2 subgroups （Ⅱ-2 and Ⅱ-3）. The lines derived from Huazhan （or genetic closely related lines） showed a genetic homogenization phenomenon. Therefore, the Huazhan-derived lines should be further improved by the utilization of germplasms with good quality, high resistance and wide adaptability from Southern China to widen the GD between Huazhan derived lines and the male sterile subgroup developed by Hunan Longping High-tech Seed Science Research Institute Co., Ltd., so as to further promote the heterosis level between the groups.

利用48对SSR引物对50份中籼型两系杂交水稻骨干亲本或改良系进行了遗传多样性分析。结果表明,基于SSR分子标记的遗传聚类结果与供试材料的系谱关系高度一致,不育系和恢复系明显聚类为2个群组(I和II),2个群组内的平均遗传距离差异不明显。湖南隆平高科种业科学研究院有限公司选育的多数不育系材料聚合为不育系群组下的一个单独亚组(I-4),且在4个不育系亚组中具有最大的组内平均遗传距离。华占衍生系和广东常规稻独立于9311衍生系或近缘系,且已明显分化为2个亚组(II-2和II-3)。华占衍生系或近缘系组存在一定程度的同质化问题,为进一步拓展此群组与湖南隆平高科种业科学研究院有限公司不育系群组之间的遗传距离,提高群组间的杂种优势水平,需继续引入华南优质、高抗、广适种质进行改良。