摘要
采用购于河北安国的当归种子,将其经过赤霉素处理后萌发的根尖为材料,使用显微摄影技术与常规的染色体制片方法对当归的细胞染色体进行核型分析。结果显示:当归数目为2 n=22,与前人的研究结果相同;核型公式为2 n=22=6 M+14 m+2 sm,未见随体,核型为2A型,核型不对称系数(AS.K%)为54.85%。染色体总长度为113.4μm,染色体总体积为83.21μm^3。
The seeds of tested Angelica sinensis （Oliy.）Diels were purchased from the planting base of Hebsi Anguo Traditional Chinese Medicine Market. With the root tips of germinated Angelica sinensis seeds treated with GA3 as materials, karyotype analysis was performed by photomicrographic technology and conventional chromosome preparation method. The results showed that the chromosome number of A. sinensis was 2 n=2.2, which accords with previous studies; and no satellites were observed, and the chromosome karyotype was 2A, with the asymmetrical karyotype coefficient （AS.K%） of 54：85%. The total length of the chromosomes was 113.4 pro, and total volume of chromosomes was 83.21 μm3.
作者
何永杰
刘芳
裴毅
聂江力
吴姿锐
杨雪君
Yongjie HE Fang LIU Yi PEI Jiangli NIE Zirui WU Xuejun YANG(College of Horticulture and Landscape , Tianjin Agricultural University, Xiqing 300384, China Tianjin Cuiping Lake Science Park, Jizhou 301908, China)
基金
Supported by Tianjin Municipal Science and Technology Project(16KPXMSF00160,16PTZSTG00020)~~
关键词
当归
染色体
核型分析
Angelica sinensis （Oliy.） Diels
Chromosome
Karyotype analysis
