氯吡格雷对幽门螺杆菌感染小鼠胃黏膜损伤的影响

Research of the effect of clopidogrel on gastric mucosal injury in mice infected with Helicobacter pylori

目的:观察氯吡格雷对感染幽门螺杆菌(Hp)的小鼠胃黏膜损伤的影响。方法:BALB/c小鼠30只,随机分为对照组、Hp组、(氯吡格雷+Hp)组,10只/组。先将Hp组、(氯吡格雷+Hp)组小鼠进行Hp造模,造模完成后,将(氯吡格雷+Hp)组进行氯吡格雷灌胃,Hp组进行生理盐水灌胃,处死小鼠,按Guth法进行大体评分;采用免疫组化法对Claudin、肿瘤坏死因子(TNF-α)进行定性分析。结果:Hp组、(氯吡格雷+Hp)组小鼠胃黏膜大体评分高于对照组(P<0.05),(氯吡格雷+Hp)组大体评分高于Hp组(P<0.05);免疫组化结果表明,与对照组相比,Hp组、(氯吡格雷+Hp)组Claudin低表达,(氯吡格雷+Hp)组表达水平最低(P<0.05);与对照组相比,Hp组、(氯吡格雷+Hp)组TNF-α高表达,(氯吡格雷+Hp)组表达水平最高(P<0.05)。结论:Hp对小鼠胃黏膜有损伤;氯吡格雷加剧Hp感染小鼠胃黏膜损伤;Claudin参与氯吡格雷作用下的Hp感染小鼠胃黏膜损伤的形成,氯吡格雷通过炎症反应因子TNF-α高表达而加重对已感染Hp小鼠的胃黏膜损伤,表明炎症反应在其中起了重要作用。

Objective：To observe the effect of clopidogrel on gastric mueoeal injury in mice infected with Helicohacter pylori （Hp）. Methods ：30 BALB/c mice were randomly divided into control group, Hp group, clopidogrel ＋ Hp group, 10 mice in each group. Two experimental groups were first made up model of Hp, after the completion of the model, the clopidogrel ＋ Hp group was given intragastric administration of clopidogrel, while the Hp group was given intragastrie administration of saline. Then the mice were killed, Gross score evaluated by Guth method; immunohistechamistry of Clandin and TNF-α for qualitative analysis was conducted. Results ： （ 1 ） The gastric mucasa score in mice in the experimental group was higher than that of the control group （ P 〈0.05 ） ; the gross score of the Clopidogrel ＋ Hp group was higher than that of Hp group （ P 〈 0.05 ） ; （ 2 ） Imraunohistochemistry （Clandin） ： compared with the control group, Claudin protein in the experimental groups was expressed at low levels （ P 〈 0.05 ） ; the expression of Clopidagrel ＋ Hp group was the lowest （ P 〈 0.05 ） ; （3） lrnmunohistochemistry （ TNF - α ） ： compared with the control group, TNF - α in the experimental grups was expressed at high levels （ P 〈0.05 ） ; the expression of Clopidogrel ＋ Hp group was the highest （ P 〈 0.05 ）. Conclusion： （ 1 ） Hp can damage to gastric mucosa in mice; （2） Clopidogrel can aggravate the injury of gastric mueesa in mice with HP infection ; （3） The Clandin protein is involved in the formation of gastric mucosal injury in mice infected with HP by clopidogrel; （4） Clopidagrel is a high expression of the inflammatory factor TNF- α, which aggravates the damage to the gastric mucosa of mice infected with Hp, which indicates that inflammation plays an important role.