高通量测序分析肠易激综合征患者和健康人肠道菌群差异

High-throughput Sequencing Analysis of Intestinal Microbiota in Patients with Irritable Bowel Syndrome and Healthy Individuals

目的通过高通量测序方法分析肠易激综合征患者(Irritable Bowel Syndrome,IBS)和健康人肠道菌群差异。方法采用Illumima系统Mi Seq平台对正常组和IBS组粪便进行16S r RNA测序。结果 IBS患者的肠道菌群多样性无显著变化。在门水平上,Firmicutes丰度显著增加(P<0.01),Bacteroidete的丰度和Bacteroidetes/Firmicutes的比值下降。在科水平上,正常组和IBS组粪便肠道菌群中检测到Cryomorphaceae、Erysipelotrichaceae、Campylobacteraceae和Fibrobacteraceae,且丰度变化具有显著差异(P<0.05)。结论 IBS患者和健康人相比,肠道菌群多样性未见明显差异。但某些细菌菌种存在显著差异。

To compare differences of intestinal microbiotabetween IBS patients andhealthy by using high-throughput sequencing technology. Methods The feces were collected IBS group andhealthy group. The community structure and abundance of intestinal microbiota were examined via analyzing 16S rRNA high throughput sequencing using Illumima System. Results The diversifies of intestinal bacteria were no significant differences in IBS group. In phyla level, the abundance of Firmicutes was significantlyincreased （P〈0.01）, while the abundance of Bacteroidetesand the ratio of Bacteroidetes/Firmicutes were decreased （P〈0.01）. Besides, in family level, cryomorphaceae,erysipelotrichac eae. Campylobacteraceae and fibrobacteraceae were detected in IBS group and healthy group.Their abundance were significantly difference, respectively （P〈0.05）. Conclusion No significantdifferences in the bacterial diversity indices are found, but some bacterial species significantly differbetween the patients with IBS and healthy controls.