姜黄素治疗大鼠脑出血后脑葡萄糖代谢变化的18F-FDG microPET/CT显像观察

Protective effect of curcumin on glucose metabolism evaluated by 18 F-FDG microPET/CT in rat models of intracerebral hemorrhage

目的 采用小动物PET/CT（microPET/CT）探索姜黄素治疗脑出血后脑葡萄糖代谢变化情况,评价其治疗脑出血的价值.方法 将24只健康雄性成年SD大鼠按单纯随机抽样法分为正常对照组、姜黄素治疗组、空白对照组和假手术组,每组6只.立体定位下于右侧基底节注射胶原酶Ⅳ制备大鼠脑出血模型.分别于建模成功后6h、24h、48h、3d、5d、7d、14d向各组大鼠尾静脉注入18F-FDG（17.8±0.4）MBq,30 min后行microPET/CT脑显像,勾画血肿及周围脑组织ROI,测定ROI体积和SUVmean,同时行神经生物学评分;采用重复测量资料的方差分析和Pearson相关分析处理数据.结果 姜黄素治疗组6 h^5 d的神经生物学评分较同期空白对照组低（F=183.26,P〈0.01）.MicroPET/CT显像示脑出血后血肿及周围脑组织葡萄糖摄取明显减低.血肿形成后6 h,姜黄素治疗组ROI 18F-FDG摄取减低,SUVmean 24 h时最低（1.20±0.08）,后逐渐增高,但均低于同期假手术组（F=7306.74,P〈0.01）,而高于空白对照组（F=471.50,P〈0.01）.姜黄素治疗组ROI各时间点SUVmean与相应时间点ROI体积、大鼠神经生物学评分均呈负相关（r值：-0.672和-0.727,均P〈0.05）.结论 Micro-PET/CT图像可较好地反映脑出血后血肿及周围脑组织的葡萄糖摄取减低情况,而姜黄素可改善此低代谢情况.

Objective To investigate the effect of curcumin on brain glucose metabolism in rat models of intracerebral hemorrhage （ ICH）, and evaluate the therapeutic effect of curcumin. Methods Twenty-four healthy adult male SD rats were randomly divided into 4 groups （ 6 rats/group） by simple random sampling method：normal group （ group A） , ICH＋curcumin group （ group B） , ICH ＋vehicle group （ group C） , and sham operated group （group D）. ICH model was made by injecting collagenase （2 μl） into the right cau-date nucleus of rat. 18F-FDG with a dose of （17.8±0.4） MBq was injected through caudal vein at 6 h, 24 h, 48 h, 3 d, 5 d, 7 d, 14 d after the model was built successfully. 18F-FDG microPET/CT was performed 30 min post injection at each time point. ROI in the hematoma and peri-hematoma brain tissue was drawn, and its volume and SUVmean were measured and analyzed. Meanwhile, each rat was evaluated by neurological severi-ty scores （ NSS） . Analysis of variance for repeated measurement data and Pearson correlation analysis were used. Results NSS in group B were lower than those in group C from 6 h to 5 d （F=183.26, P〈0. 01）. MicroPET/CT showed decreased glucose uptake in the hematoma and peri-hematoma brain tissue after cere-bral hemorrhage. In group B, the 18 F-FDG uptake in peri-hematoma brain tissue of ICH decreased after 6 h, and reached the minimum at 24 h （1.20±0.08）, and then increased. The glucose metabolism in group B was significantly lower than that in group D at each time point （F=7306.74, P〈0.01）, and significantly higher than that in group C （ F=471.50, P〈0.01） . SUVmean within ROI had a significantly negative correla-tion with both ROI volume and NSS in group B at each time point（ r values：-0.672 and -0.727, both P〈0. 05） . Conclusions MicroPET/CT might visualize decreased glucose uptake of hematoma and peri-hema-toma brain tissue after cerebral hemorrhage. Curcumin might have a neuroprotective effect on ICH, and im-prove the glucose uptake in hematoma and peri-hematoma brain tissue.