摘要
目的对9个产地夏枯草的主要化学成分进行测定,并进行主成分分析和聚类分析。方法用分光光度计法测定夏枯草多糖和总黄酮含量,HPLC法测定迷迭香酸含量。结果 9个产地夏枯草多糖含量在6.81%~12.49%,总黄酮含量在3.29%~5.82%,迷迭香酸含量在1.0%~3.3%。夏枯草的主成分为多糖,聚类结果显示地理位置相近的聚为一类。结论 9个产地夏枯草的主要化学成分含量比较,差异具有统计学意义,建议夏枯草质量评价应结合多糖含量。
Objective To determine the main chemical components of the Prunella vulgaris L. collected from nine different places and carry out the principal component analysis and cluster analysis. Methods The polysaccharides and total flavonoids of Prunella vulgaris L. were determined by spectrophotometer and the rosmarinic acid was determined by HPLC. Results The content of polysaccharides, total flavonoids and rosmarinic acid in the Prunella vulgaris L. collected from nine different places was 6.81%- 12.49 %, 3.29 %- 5.82%, and 1.0%-3.3% respectively. The polysaccharides proved to be the main chemical component of Prunella vulgaris L. The results of clustering analysis showed that the Prunella vulgaris L. with geographical proximity could be clustered together. Conclusion There are significant differences among the Prunella vulgaris L. collected from nine different places in the main chemical components, so it is suggested that the conten of Prunella vulgaris L. should be considered in the quality the quality evaluation of Prunella vulgaris L.
作者
徐锐
张雪绒
郭巧生
刘丽
赵淼
秦琴
陈宇航
Xu Rui Zhang Xuerong Guo Qiaosheng Liu Li Zhao Miao Qin Qin Chen Yuhang(School of Pharmacy, Chengdu Medical College, Chengdu 610083, China Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210059, China)
出处
《成都医学院学报》
CAS
2017年第5期551-554,566,共5页
Journal of Chengdu Medical College
基金
国家自然科学基金项目(No:31500263
30772730
81072986)
国家中医药管理局中医药行业科研专项(No:201507002)
中国博士后科学基金(No:2014M560726
2016T90869)
国家级大学生创业项目(No:201413705026)
成都医学院大学生创新实验项目(No:CXXS201417)
关键词
夏枯草
多糖
总黄酮
迷迭香酸
主成分分析
聚类分析
Prunella vulgaris L.
Polysaccharides
Total flavonoids
Rosmarinic acid
Principal component analysis
Cluster analysis
