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ELISA试剂盒在奶牛早期妊娠检测中的应用

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摘要 准确、快速、操作便捷的奶牛早期妊娠诊断技术是规模化奶牛场提高繁殖效率和养殖经济效益的重要手段。本试验通过采用ELISA试剂盒(牛快速可视孕检试剂盒),在2016年3月~2017年2月期间,对北京市房山区某规模化牛场的荷斯坦奶牛的168头份血样进行检测,并将检测结果与55~60d的直肠检查结果进行比较。结果表明,不同胎次奶牛的空怀检测准确率为97.68%~98.52%,怀孕检测准确率为98.51%~98.83%。ELISA检测作为奶牛早期妊娠诊断的措施,对规模化牛场具有重大的意义。
出处 《山东畜牧兽医》 2017年第10期12-13,共2页 Shandong Journal of Animal Science and Veterinary Medicine
基金 北京农业职业学院技术研发与示范推广基金项目(XY-YF-16-09)
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  • 1Ayad A,Sousa N M,Sulon J,et al. Comparison of five radioimmunoassay systems for PAG measurement: ability to detect early pregnancy in cows.[J]. Reprod Domest Anita,2007,42(4) : 433-440.
  • 2Butler J E, Hamilton W C,Sasser R G, et al. Detection and par- tial characterization of two bovine pregnancy-specific proteins[J]. Biol Reorod.1982.26(5), 925-933.
  • 3El Amiri B,Remy B,Sousa N M,et al. Isolation and partial characterization of three pregnancy-associated glycoproteins from the ewe plaeenta[J]. Mol Reprod Dev,2003,64(2): 199-206.
  • 4E1-Battawy K A, Sousa N M, Szenci O, et al. Pregnancy-associated glycoprotein profile during the first trimester of pregnancy in Egyptian buffalo cows[J]. Reprod Domest Anita,2009,44(2): 161-166.
  • 5Friedrich M, Holtz W. Establishment of an ELISA for measuring bovine pregnancy-associated glycoprotein in serum or milk and its application for early pregnancy detection[J]. Reprod Domest Anim,2010,45(1) :142-146.
  • 6Gajewski Z, Melo De Sousa N, Beckers J F, et al. Concentration of bovine pregnancy associated glycoprotein in plasma and milk: its application for pregnancy diagnosis in cows [J]. J Physiol Pharmacol,2008,59(Suppl 9) : 55-64.
  • 7Garbayo J M, Remy B, Alabart J L, et al. Isolation and partial characterization of a pregnancy-associated glycoprotein family from the goat placenta[J]. Biol Reprod,1998,58(1) : 109-115.
  • 8Green J C, Newsom E M, Lucy M C. Incorporation of a rapid pregnancy-associated glycoprotein ELISA into a CIDR-Ovsynch resynchronization program for a 28 day re-insemination interval[J]. Theriogenology,2011,75(2): 320-328.
  • 9Green J C,Volkmann D H,Poock S E,et al. Technical note:A rapid enzyme-linked immunosorbent assay blood test for pregnancy in dairy and beef catile[J]. J Dairy Sci,2009,92(8): 381943824.
  • 10Hubbert W T. Recommendations for standardizing bovine reproductive terms by committee on bovine reproductive nomenclature [J]. Cornell Vet,1972,62: 216-237.

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