介入性热化疗对兔肝VX-2肿瘤血管渗透性的影响

Effect of Interventional Chemother motherapy on Vascular Permeability of Tumor Liver Tissue and Normal Liver Tissue in VX-2Tumor-bearing Rabbits

背景及目的:研究表明,加热对兔VX-2细胞阿霉素化疗有增敏作用,加热可提高细胞内阿霉素的含量。本研究探讨介入性热化疗对正常肝组织及肿瘤肝组织血管渗透性的影响。方法:建立可供实验用的兔VX-2移植性肝癌模型30只,随机分为3组:非灌注组(插管后只注射1%伊文思蓝),常温灌注组(灌注液为25℃生理盐水)。热灌注组(灌注液为60℃生理盐水)。使用标准曲线和分光光度法测量各组组织中伊文思蓝含量(作为血管渗透性的指标)。结果:肿瘤组织与肝组织的EB含量在3组中均有差别(P<0.05);正常灌注组与非灌注组肝组织的EB含量、肿瘤组织的EB含量无明显差别(P>0.05);热灌注组与非灌注组、正常灌注组肝组织的EB含量、肿瘤组织的EB含量有明显差别(P<0.05)。结论:介入性热化疗可以增加肝组织及肿瘤组织的血管渗透性。

Background &Objective:It was reported that heating could en hance the sensitivity of chemothera py with Adriamycin and increase the intracellular content of Adriamycin.The aim of this study was to investigate the effect of interventional chemothermotherap y on vascular permeability of tumor l iver tissue and normal liver tissue i n VX-2tumor-bearing rabbits.Methods:Thirty rabbits used as implanted hep atocarcinoma model were randomly di vided into 3groups:non-perfusion group(injected only with 1%Evans blue afte r catheterization),normothermic perfusion group(the perfusion fluid was 25℃normal solut ion),and hyperthermic perfusion group(the perfusion fluid was 60℃normal solution).The contents of Evans blue in the tis sues of three groups,which were used as the indices of vascular permeabil ity,were calculated by the standard curv e and spectrophotometry.Results:The Evans blue contents in tumor live r tissue and normal liver tissue is statistically different (P<0.05).There was no overt difference of the Evans blue contents in two kinds of tissue between normal perfu sion group and non-perfusion group.There was overt difference of the Evans blue contents in two kinds of tissue between hyperthermic perfusion group and normothermic perfusion group.Conclusion:Interventional chemothermotherapy could increase the vascular permeability of normal liver tissue and tumor liver t issue.