人源α-synuclein跨细胞转运特性及对神经细胞突起的影响

Transfer of human derived α-synuclein across cell to cell and effects on neurite

目的确定人源α-synuclein是否能够跨细胞转运,过表达人源α-synuclein对神经细胞突起有何影响。方法建立稳定表达人源α-synuclein的N2a细胞株,通过接触和非接触两种方法观察人源α-synuclein的转运现象,通过测量神经突起长度,评价人源α-synuclein过表达对神经细胞突起生长的影响。结果接触和非接触两种方法均能够观察到人源α-synuclein跨细胞转运,但总体转运率低。过表达人源α-synuclein导致神经细胞突起生长不良。结论人源α-synuclein跨细胞转运现象存在,转运可能受到多因素影响,效率较低。过表达人源α-synuclein蛋白对神经突起有损伤作用。

Objective To confirm whether human derived a-synuclein have ability to transfer across cell to cell. To evaluate the effect of overexpression of human derived a-synuclein on the neurite morphology. Methods Stable N2a cell lines overexpressed human derived α-synuclein were established. Contact and non-contact culture methods were used to investigate the transfer of human derived α-synuclein in vitro. The length of neurite was measured during overexpression of human derived α-synuclein. Results The transfer of α-synuclein could be observed through the contact culture and noncontact culture. However,the efficiency of α-synuclein transfer was low in vitro. Additionally,high expression of human derived α-synuclein inhibited the neurite growth. Conclusion A little of human derived α-synuclein transfers from cell to cell in vitro. Over expression of human derived α-synuclein damages the neurite.