人参多糖与奥沙利铂联合作用对肝癌细胞的生物学行为的影响

Effects of Ginseng Polysaccharide Combined with Oxaliplatin on Biological Behavior of Hepatocellular Carcinoma Cells

目的探讨人参多糖与奥沙利铂联合作用对肝癌细胞的生物学行为的影响。方法细胞活性检测人参多糖联合奥沙利铂对肝癌细胞的影响;Transwell侵袭实验检测加入姜黄素和卡铂对肺癌细胞的侵袭能力的影响;裸鼠成瘤实验检测人参多糖联合奥沙利铂对肝癌细胞的成瘤能力的影响;Western blotting检测人参多糖联合奥沙利铂促进凋亡相关蛋白在肝癌细胞中的表达。结果人参多糖和奥沙利铂联合用药对肝癌细胞的抑制效果明显增加;在人参多糖和奥沙利铂联合治疗组中,HepG2肝癌细胞侵袭能力减弱,肿瘤重量显著降低;人参多糖和奥沙利铂联合应用可以激活下游凋亡蛋白活性从而影响肝癌细胞的生物学活性。结论人参多糖和奥沙利铂联合用药可以通过下调凋亡蛋白水平抑制HepG2肝癌细胞株的侵袭能力和成瘤能力。

Objective To investigate the effect of ginseng polysaccharide combined with oxaliplatin on the biological behavior of HCC cells.Methods The effect of ginseng polysaccharide combined with oxaliplatin on hepatocarcinoma cells was detected by cell viability.Transwell invasion assay was used to detect the effect of curcumin and carboplatin on the invasion of lung cancer cells.The effect of ginseng polysaccharide combined with oxaliplatin on tumorigenic ability of hepatoma cells was observed in nude mice.Western blotting was used to detect the expression of apoptosis-related proteins in hepatocarcinoma cells induced by ginseng polysaccharide combined with oxaliplatin. Results The inhibitory effect of ginseng polysaccharide and oxaliplatin on hepatocarcinoma cells was significantly increased. The combination of ginseng polysaccharide with oxaliplatin weakened the invasion ability of HepG2 hepatoma cells,reduced the tumor weight significantly,and activated the downstream apoptotic protein,thus affecting the biological activity of hepatocarcinoma cells.Conclusion The combination of ginseng polysaccharide and oxaliplatin can inhibit the invasion and tumorigenic ability of HepG2 hepatoma cell line by down-regulating the level of apoptosis protein.