白介素4、10、12、13、IFN-γ、TGF-β在不同时期特应性皮炎病人血清中的变化

Changes of IL-4,IL-10,IL-12,IL-13,IFN-γ and TGF-β in the serum of atopic dermatitis patients at different stages of the disease

目的研究疾病发作期和缓解期特应性皮炎(atopic dermatitis,AD)病人血清中白细胞介素-4(interleukin-4,IL-4)、IL-10、IL-12、IL-13、干扰素-γ(interferon-γ,IFN-γ)、转化生长因子-β(transforming growth factor-β,TGF-β)等细胞因子的变化,探讨其在AD发病机制中的作用。方法收集疾病发作期AD病人79例,其中经治疗皮损缓解的AD病人(缓解组)40例,未缓解的AD病人(未缓解组)39例。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)测定病人治疗前后血清中IL-4、IL-10、IL-12、IL-13、IFN-γ、TGF-β的浓度。结果缓解组AD病人治疗后血清中IL-4、IL-12、IL-13、IFN-γ与治疗前相比,差异均有统计学意义(均P<0.05)。缓解组AD病人治疗后血清中IL-10、TGF-β与治疗前相比,差异无统计学意义(P>0.05)。未缓解组AD病人治疗后血清中IL-4、IL-10、IL-12、IL-13、IFN-γ、TGF-β与治疗前相比,差异均无统计学意义(P>0.05)。结论 AD病人病情缓解时血清中IL-4、IL-13表达显著下调,IFN-γ和IL-12表达显著升高。

Objective To investigate the changes of interleukin-4（IL-4）,IL-10,IL-12,IL-13,interferon-γ（IFN-γ） and transforming growth factor-β（TGF-β） in the serum of atopic dermatitis patients at different stages of the disease,and explore their significance.Methods Seventy-nine atopic dermatitis patients at acute stage were enrolled. There were 40 cases at remission stage（remission group,RG）,and 39 cases were still at acute stage after treatment（acute group,AG）. The serum levels of IL-4,IL-10,IL-12,IL-13,IFN-γ and TGF-β were detected by enzyme-linked immunosorbent assay（ELISA） before and after treatment. Results There were significant differences in the serum levels of IL-4,IL-12,IL-13 and IFN-γ before and after treatment in the remission group（P〈0. 05）. There were no significant differences in the serum levels of IL-10,TGF-β before and after treatment in the remission group（P〉0. 05）. There were no significant differences in the serum levels of IL-4,IL-10,IL-12,IL-13,IFN-γ and TGF-β before and after treatment in the acute group（P〉0. 05）. Conclusion Lower expression of IL-4,IL-13,and higher expression of IFN-γ,IL-12 in serum at remission stage of atopic dermatitis.