一种水溶性酞菁纳米晶的制备及光敏活性研究

Preparation and Photodynamic Activity of a Water-Soluble Phthalocyanine Nanoparticle

通过"再沉淀法"将一种疏水性的锌酞菁(2(3),9(10),16(17),23(24)-四-(((2-(三苯甲基-氨基)乙胺基)甲基)苯氧基)锌酞菁(Ⅱ),NNZnPc)转变成一种水溶性的酞菁纳米晶(NNZnPc-NP),该酞菁纳米晶符合光动力疗法(Photodynamic therapy,PDT)对药物运载的需求.通过透射电镜(TEM)、紫外-可见分光光度计(UV-Vis)、倒置荧光显微镜研究和表征了该酞菁纳米晶的表面形貌、稳定性、单线态氧产生能力以及光敏抗肿瘤活性.所制备的NNZnPc-NP颗粒粒径均一、分散性好、在水溶液中能稳定存在,且有较高的单线态氧(~1O_2)产生效率及抗光漂白的能力.体外抗肿瘤实验结果表明,可见光照射下NNZnPc-NP可有效杀灭宫颈癌细胞(HeLa),有较好的应用潜力.

A novel water soluble nanocrystalline phthalocyanine （ NNZnPc-NP） was prepared using reprecipitation method.The surface morphology, stabi l i ty, singlet oxygen generation abi l i ty and photosensitive anticancer act ivity of it were studied by transmission electron microscopy（TEM） ,UV-Vis spectrophotometer and inverted fluorescence microscope. The results showed that the particle size of NNZnPc-NP is uniform, the dispersion is good,and it can be stable in aqueous solution. Besides, it also exhibits excellent singlet oxygen generation abi lity and photostability. The in vitro anticancer experiment results confirm that NNZnPc-NP can ef f icient ly kill HeLa cells under the visible l ight irradiation. The above results indi-cate NNZnPc has good potential to be used as cl inical photosensitizer for PDT.