忽地笑多糖分子量及单糖组成研究

Molecular Weight and Monosaccharide Composition of Polysaccharide from Lycoris aurea

研究忽地笑多糖PBL1、PBL2和PBL3的分子量和单糖组成。分别采用凝胶排阻色谱-多角度激光光散射-示差检测法(GPC-MALLS-RI)和高效液相法(HPLC)测定忽地笑多糖的分子量及单糖组成。PBL1的重均分子量(Mw)为3.642×10~3g/mol;PBL2的Mw为1.206×10~4g/mol;PBL3的Mw为3.992×10~5g/mol。多糖PBL1由D-Man、D-Glu和D-Gal组成,摩尔比为1∶0.34∶0.036;多糖PBL2由D-Man、Rha、D-Gal UA、D-Glu、D-Gal和Ara组成,摩尔比为1∶0.29∶0.78∶21.89∶2.69∶0.63;多糖PBL3由D-Man、Rha、D-Glu UA、D-Gal UA、D-Glu、D-Gal和Ara组成,摩尔比为1∶2.63∶0.47∶1.63∶0.83∶6.92∶1.34。忽地笑多糖PBL1、PBL2和PBL3均主要由D-Man、D-Glu和D-Gal组成,它们的分子量大小差异明显。

To Study the molecular weight and monosaccharide composition in polysaccharide from PBL1,PBL2 and PBL3.The molecular weight and monosaccharide composition in polysaccharide were determined by GPC-MALS-RI and HPLC.The results showed that the average molecular weight （Mw） of PBL1-1,PBL2 and PBL3 were 3.642×103,1.206×104 and 3.992×105 g/mol,respectively.PBL1-1 consisted of D-Man,D-Glu and D-Gal with the mole ratio of 1∶0.34∶0.036.PBL2 was composed of D-Man,Rha,D-Gal UA,D-Glu,D-Gal and Ara with the mole ratio of 1∶0.29∶0.78∶21.89∶2.69∶0.63.PBL3 was composed of D-Man,Rha,D-Glu UA,D-Gal UA,D-Glu,D-Gal and Ara with the mole ratio of 1∶2.63∶0.47∶1.63∶0.83∶6.92∶1.34.The polysaccharide of PBL1,PBL2 and PBL3 from Lycoris aurea consisted of D-Man,D-Glu and D-Gal,which had considerable differences in molecular weight.