羊耳菊活性部位提取物在大鼠胆汁中代谢产物的研究

Metabolism of Active Fractions of Inula Cappa in Rat Bile

采用超高效液相色谱-四极杆串联飞行时间质谱(UHPLC/Q-TOF MS/MS)技术,分析大鼠灌胃羊耳菊活性部位提取物后,主要成分在大鼠胆汁中的排泄方式。SD大鼠胆管插管手术后,灌胃给予羊耳菊活性部位提取物(100 g/kg生药量),收集给药后12 h的胆汁样品。样品经正丁醇液液萃取法处理后,采用UHPLC/Q-TOF MS/MS对胆汁中的主要代谢产物的结构进行分析推测。实验结果显示,胆汁中共检测到27个代谢产物,样品中检测到大量的咖啡酰基奎宁酸还原,甲基化,葡萄糖醛酸化和甲基葡萄糖醛酸化代谢产物,以咖啡酰基奎宁酸的甲基葡萄糖醛酸化为主。说明大鼠口服羊耳菊提取物后,成分可通过胆汁进行消除,并且甲基葡萄糖醛酸结合物是主要的物质形式。

To establish an ultra-performance liquid chromatography/quadrupole time-of-flight mass （UHPLC/Q-TOF MS/MS） method for analyzing the metabolites of main component in Inula cappa extract in rat bile after oral administration.Cannulas were surgically inserted into the bile duct of SD rats and bile was collected for 12 h after oral administration of active fractions of I.cappa at a dose of 100 g/kg of the material.After n-butyl alcohol precipitation treatment,the chemicals and their metabolites in rat bile were identified using UHPLC/Q-TOF MS/MS with selected reaction monitoring mode.The results revealed that twenty-seven metabolites were determined in bile,main metabolites are hydrogenation,methylation and glucuronidation of caffeoylquinic acid.It indicated that the chemicals can occur biotransformation in bile,the metabolic pathways mainly are methylation and demethylation glucuronidation.