STC-1基因在大、小体型猪中表达与分布的差异分析

Expression and Distribution Difference Analysis of STC-1 Gene in Big and Small Body Shape Pigs

试验分别采集40日龄小体型猪(巴马猪)和大体型猪(大白猪)的心脏、肝脏、脾脏、肺脏、肾脏、头骨、骨骼肌组织,利用实时荧光定量PCR检测斯钙素-1(stanniocalcin 1,STC-1)基因mRNA在各个组织中的表达水平,并通过Western blotting检测STC-1蛋白在各个组织中的分布。实时荧光定量PCR检测结果表明,STC-1基因mRNA在巴马猪和大白猪肺脏、肾脏中相对表达水平较高,在骨骼肌中的表达水平最低;除心脏和骨骼肌外,巴马猪其余各组织中STC-1基因mRNA表达水平均显著高于大白猪(P<0.05)。Western blotting检测结果表明,巴马猪肝脏中STC-1蛋白的表达量最高,而大白猪脾脏中STC-1蛋白表达量最高,两者差异显著(P<0.05);巴马猪肺脏、肝脏、骨骼肌及心脏组织中STC-1蛋白表达量均极显著高于大白猪(P<0.01);而巴马猪肾脏、脾脏中STC-1蛋白表达量极显著低于大白猪(P<0.01)。本研究首次对大、小体型猪不同组织的STC-1基因mRNA表达水平及其STC-1蛋白分布进行检测,导致该基因表达与分布差异的原因可能与两种猪受外界环境应激及生长发育差异有关。

bThe heart,liver,spleen,lung,kidney,skull,skeletal muscle were collected from 40-day-old Bama Mini pig and Large White pig,stanniocalcin 1（ STC-1） gene mRNA and STC-1 protein were detected with Real-time PCR and Western blotting methods,respectively. The Real-time PCR results showed that the expression level of STC-1 gene mRNA was higher in lung and kidney,but was the lowest in skeletal muscle of Bama Mini pig and Large White pig. Except for heart and skeletal muscle tissues,the expression level of STC-1 gene mRNA in other tissues of Bama Mini pig was significantly higher than Large White pig（P〈0.05）. The Western blotting results showed that the STC-1 protein distribution were the highest in liver and spleen of Bama Mini pig and Large White pig,respectively,and the difference was significant（P〈0.05）. The STC-1 protein expression in lung,liver,skeletal muscle and heart of Bama Mini pig were extremely significantly higher than Large White pig（ P〈0. 01）,and the STC-1 protein expression in kidney and spleen of Bama Mini pig were extremely significantly lower than Large White pig（ P〈0. 01）. STC-1 gene mRNA and protein were firstly detected in different tissues from big and small body shape pigs,this might have a relationship with various external environments stress and development.