促卵泡素和促黄体生成素对儋州鸡生殖激素调控规律的研究

Study on Regulation of Reproductive Hormones in Danzhou Chicken by FSH and LH

为研究促卵泡素(FSH)和促黄体生成素(LH)对儋州鸡体内其他生殖激素的调控规律,本试验通过改变FSH和LH在儋州鸡血液中的浓度,并采用双抗体一步夹心法酶联免疫吸附试验(ELISA)对处理前后儋州鸡血液中FSH、LH、催乳素(PRL)、孕酮(P)、雌二醇(E2)的浓度进行测定。结果发现,注射外源性FSH和LH分别能提高儋州鸡血液中FSH和LH浓度;当儋州鸡血液中FSH或LH浓度显著升高时则均能引起PRL浓度显著降低(P<0.05),但当FSH和LH浓度同时显著升高时,PRL浓度显著升高(P<0.05);当儋州鸡血液中FSH浓度显著升高时,E2及P浓度显著提升(P<0.05),且在高浓度LH的协同下提升幅度更大;当儋州鸡血液中LH浓度显著升高时E2及P浓度升高但不显著(P>0.05)。本研究结果表明,儋州鸡血液中FSH或LH浓度的提高均能降低PRL的浓度,并能不同程度的提升E2及P的浓度,但FSH与LH浓度同时提高则能通过协同作用刺激E2及P浓度的大幅提升,当E2及P浓度过高时能通过刺激PRL的释放,负反馈调节血液中FSH与LH,并恢复血液中E2及P浓度。

To investigate the regulation of reproductive hormones in Danzhou chicken by follicle-stimulating hormone（ FSH） and luteotropic hormone（ LH）,the concentration of FSH and LH in Danzhou chicken blood were changed by different treatments and the concentration of FSH,LH,prolactin（ PRL）,progesterone（ P）,and estradiol（ E2） in the blood were determined using the enzyme-linked immunosorbent assay（ ELISA）.The results showed that exogenous FSH and LH could improve the concentrations of FSH and LH,respectively. When one of FSH and LH increased alone,the concentration of PRL was significantly decreased（P〈0.05）,while it was significantly increased when FSH and LH were both increased（P〈0.05）.The high level of the FSH could result the significant increase of E2 and P（ P〈0.05）,and it would expand the increasing effect in cooperation with the high concentration of LH. While the high level of the LH could elevate the concentration of E2 and P,but effect was not significant（P〈0.05）.In conclusion,the study suggested that the increase level of FSH or LH could reduce the concentration of PRL in Danzhou chicken,meanwhile,it could increase the concentration of E2 and P in varying degrees.However,both of the FSH and LH increase in the blood could result the significant increase of E2 and P as the synergy. When the concentration of E2 and P were too high,it could stimulate the release of PRLwhich could adjust the concentration of FSH and LH,and rebalance the concentrations of E2 and P in the blood.